Fields »

SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane

Additional location(s)

Nucleoli, Nucleus but not nucleoli 

Staining summary

Mainly localized to the plasma membrane but also to the nucleus.

Reliability (APE)

Medium

Antibodies in assay

HPA005830, CAB010896
 

Antibody HPA005830

 

Antibody CAB010896

 

         Cell line A-431

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Location(s)

  Nucleus but not nucleoli 

Staining characteristics

   

Staining intensity

  Strong 

Validation

  
Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
 

Comment

   

         Cell line U-2 OS

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Location(s)

  Nucleus but not nucleoli, Plasma membrane 

Staining characteristics

   

Staining intensity

  Moderate 

Validation

  
One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Nucleoli Nucleus but not nucleoli, Plasma membrane 

Staining characteristics

Cluster, Spotty  

Staining intensity

Moderate Moderate 

Validation

Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nucleoli.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & plasma membrane.
 

Summary

Staining of clusters of spots in nucleoli in U-251MG.   Staining of nucleus in all three cell lines with additional staining of plasma membrane in U-2 OS and U-251 MG.  

Validation

The subcellular location is not consistent with literature.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Nucleoli
Nucleus but not nucleoli
Plasma membrane