ATG9A

ANTIBODY AND ANTIGEN INFORMATION

? »
 

Antibody HPA059551

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA059551 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

12 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

57 

Antigen sequence

IYNICCYWEIHSFYLHALRIPMSALPYCTWQEVQARIVQTQKEHQICIHK
RELTELD
 

Matching transcripts

ATG9A-001 - ENSP00000379983 [100%]
ATG9A-002 - ENSP00000386482 [100%]
ATG9A-003 - ENSP00000355173 [100%]
ATG9A-004 - ENSP00000386710 [100%]
ATG9A-010 - ENSP00000396404 [100%]
ATG9A-021 - ENSP00000386535 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:30 

Validation IH

Uncertain: One antibody yielding a staining pattern and there is no available gene/protein characterization data or the staining pattern is partly consistent with or contradicted by limited gene/protein characterization data 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

94.4, 87.4, 60.7, 15.6 

Antibody dilution

1:80 

Validation WB

Uncertain: No bands detected 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:500 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

? »
 
 
 
ATG9A-001
 
ATG9A-002
 
ATG9A-003
 
ATG9A-004
 
ATG9A-010
 
ATG9A-021