The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human kidney shows distinct positivity in glomerular cells.
Immunohistochemical staining of human kidney shows membranous positivity in glomeruli.
Immunohistochemical staining of human kidney shows strong positivity in cells in glomeruli.
Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in white pulp.
Immunohistochemical staining of human tonsil shows distinct reticular positivity in germinal center.
Summary
Strong cytoplasmic positivity with a reticular pattern was observed in lymphatic tissues. Renal glomeruli showed strong membranous immunoreactivity. Remaining normal tissues were negative.
Highly selective cytoplasmic staining of glomeruli in kidney, white pulp in spleen and germinal center in tonsil. Virtually no staining in other tissues.
Macrophages of lung, gastrointestinal tract, exocrine pancreas, leydig cells, lymphoid tissues and renal glomeruli showed moderate to strong cytoplasmic positivity. Distinct reticular pattern was observed in lymphoid germinal centers. Remaining normal tissues were weakly stained or negative.
Lymphoid tissues exhibited moderate to strong cytoplasmic staining with distinct reticular pattern in germinal centers. Renal glomeruli were distinctly stained. Respiratory and squamous epithelia, gastrointestinal tract, exocrine pancreas, glandular cells in female genital tract and breast showed moderate cytoplasmic positivity with a granular pattern. Remaining normal tissues were mainly negative.
Glomeruli of kidney, fraction of bone marrow hematopoietic cells and reticular cells in lymphoid tissues were distinctly stained. Most remaining normal tissues were negative.
Validation
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are consistent with gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are consistent with gene/protein characterization data
The multi-targeting antibody yielding a staining pattern consistent with gene/protein characterization data for at least one of the genes whereas any of the other genes has no available gene/protein characterization data or is contradicted by gene/protein
