Fields »

SUBCELLULAR LOCATION

? »

Main location(s)

Plasma membrane, Cytoskeleton (Intermediate filaments)

Additional location(s)

Cytoplasm, Cell Junctions 

Staining summary

Staining of plasma membrane and cytoskeleton (intermediate filaments) in all three cell lines. Additional staining of cytoplasm in A-431 and U-2 OS. Additional staining of cell junctions in A-431.

Reliability (Single)

Antibodies in assay

HPA028985
 

Antibody HPA028985

 

         Cell line A-431

Toggle channels

 

Location(s)

Plasma membrane, Cytoplasm, Cytoskeleton (Intermediate filaments), Cell Junctions 

Staining characteristics

 

Staining intensity

Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

Toggle channels

 

Location(s)

Plasma membrane, Cytoplasm, Cytoskeleton (Intermediate filaments) 

Staining characteristics

 

Staining intensity

Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

Toggle channels

 

Location(s)

Plasma membrane, Cytoskeleton (Intermediate filaments) 

Staining characteristics

 

Staining intensity

Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in plasma membrane, cytoplasm, cytoskeleton (intermediate filaments) & cell junctions.
 

Summary

Staining of plasma membrane and cytoskeleton (intermediate filaments) in all three cell lines. Additional staining of cytoplasm in A-431 and U-2 OS. Additional staining of cell junctions in A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cell junctions
Cytoplasm
Intermediate filaments
Plasma membrane