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SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Nucleus but not nucleoli, Endoplasmic reticulum

Additional location(s)

 

Staining summary

Staining of endoplasmic reticulum and speckled staining of nucleus in all three cell lines.

Reliability (Single)

Antibodies in assay

HPA001312
 

Antibody HPA001312

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Endoplasmic reticulum 

Staining characteristics

Speckle 

Staining intensity

Moderate 

Validation

The multi-targeting antibody yielding a staining pattern partly consistent with available gene/protein characterization data for all of the genes.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Endoplasmic reticulum 

Staining characteristics

Granular, Speckle 

Staining intensity

Moderate 

Validation

The multi-targeting antibody yielding a staining pattern partly consistent with available gene/protein characterization data for all of the genes.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus, Endoplasmic reticulum 

Staining characteristics

Speckle 

Staining intensity

Moderate 

Validation

The multi-targeting antibody yielding a staining pattern partly consistent with available gene/protein characterization data for all of the genes.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & endoplasmic reticulum.
 

Summary

Staining of endoplasmic reticulum and speckled staining of nucleus in all three cell lines.  

Validation

The subcellular location is supported by literature.
 

Dictionary

Endoplasmic reticulum
Nucleus
Nucleus but not nucleoli