Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Nucleoli, Nucleus but not nucleoli 

Staining summary

Mainly localized to the cytoplasm. Additionally localized to the nucleus.

Reliability (APE)

Medium

Antibodies in assay

HPA018295, HPA020339, HPA021146
 

Antibody HPA018295

 

Antibody HPA020339

 

Antibody HPA021146

 

         Cell line A-431

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Location(s)

Cytoplasm Nucleus but not nucleoli, Cytoplasm Nucleoli, Cytoplasm 

Staining characteristics

 Granular Spotty 

Staining intensity

Strong Moderate Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Cytoplasm Cytoplasm Nucleoli, Cytoplasm 

Staining characteristics

  Cluster 

Staining intensity

Moderate Moderate Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Cytoplasm Nucleus but not nucleoli, Cytoplasm Nucleoli, Cytoplasm 

Staining characteristics

  Spotty 

Staining intensity

Strong Strong Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleoli & cytoplasm.
 

Summary

Staining of cytoplasm in all three cell lines.   Staining of cytoplasm in all three cell lines with additional staining of nucleus but not nucleoli in U-251 MG and A-431.   Staining of cytoplasm and spots in nucleoli in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleoli
Nucleus but not nucleoli