Fields »

SUBCELLULAR LOCATION

? »

Main location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm, Centrosome

Additional location(s)

Vesicles 

Staining summary

Localized to the nucleus, cytoplasm, plasma membrane and centrosomes.

Reliability (APE)

Low

Antibodies in assay

HPA001143, CAB016777, HPA024061
 

Antibody HPA001143

 

Antibody CAB016777

 

Antibody HPA024061

 

         Cell line A-431

Toggle channels

 
 
 

Location(s)

Nucleus but not nucleoli, Cytoplasm  Nucleus but not nucleoli, Plasma membrane, Centrosome 

Staining characteristics

Granular   

Staining intensity

Moderate Negative Strong 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
No staining.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

   

         Cell line U-2 OS

Toggle channels

 
 
 

Location(s)

Nucleus but not nucleoli, Cytoplasm, Vesicles  Plasma membrane, Centrosome 

Staining characteristics

   

Staining intensity

Weak Negative Weak 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
No staining.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

   

         Cell line U-251 MG

Toggle channels

 
 
 

Location(s)

Nucleus but not nucleoli, Cytoplasm, Vesicles  Nucleus but not nucleoli, Plasma membrane, Centrosome 

Staining characteristics

   

Staining intensity

Moderate Negative Strong 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
No staining.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows no positivity.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane & centrosome.
 

Summary

Staining of cytoplasm and nucleus in all three cell lines. Additional staining of vesicles in U-251 MG and U-2 OS.   No staining was observed in any of the three cell lines.   Staining of centrosomes and plasma membrane in all three cell lines. Additional staining of nucleus in U-251 MG and A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Centrosome
Cytoplasm
Nucleus but not nucleoli
Plasma membrane
Endosome