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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

Cytoplasm, Golgi apparatus, Vesicles 

Staining summary

Staining of nucleus and vesicles in all three cell lines. Staining of the Golgi apparatus in U-251 MG and A-431. Staining of cytoplasm in A-431.

Reliability (Single)

Antibodies in assay

HPA034655
 

Antibody HPA034655

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Golgi apparatus, Vesicles 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Vesicles 

Staining characteristics

Granular 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Golgi apparatus, Vesicles 

Staining characteristics

 

Staining intensity

Weak 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & vesicles.
 

Summary

Staining of nucleus and vesicles in all three cell lines. Staining of the Golgi apparatus in U-251 MG and A-431. Staining of cytoplasm in A-431.  

Validation

The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Golgi apparatus
Nucleus but not nucleoli
Endosome