CT47A6

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA042483

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA042483 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

10 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

28 

Antigen sequence

PEEVTKSQPEKWDEEAQDAAGEEEKEQE
 

Matching transcripts

CT47A6-001 - ENSP00000415974 [100%]
 

Other gene match

CT47A10 - ENSG00000224089 [100%]
CT47A9 - ENSG00000226600 [100%]
CT47A12 - ENSG00000226685 [100%]
CT47A11 - ENSG00000226929 [100%]
CT47A7 - ENSG00000228517 [100%]
CT47A8 - ENSG00000230347 [100%]
CT47A4 - ENSG00000230594 [100%]
CT47A3 - ENSG00000236126 [100%]
CT47A1 - ENSG00000236371 [100%]
CT47B1 - ENSG00000236446 [100%]
CT47A5 - ENSG00000237957 [100%]
CT47A2 - ENSG00000242362 [100%]
 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human testis shows strong cytoplasmic and nucleolar positivity in cells in seminiferus ducts.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:750 

Validation IH

Uncertain: The multi-targeting antibody yielding a staining pattern consistent with gene/protein characterization data for at least one of the genes whereas any of the other genes has no available gene/protein characterization data or is contradicted by gene/protein 

         Western Blot

Image

 

Description

 

Target mass (kDa)

31.3, 30.1 

Antibody dilution

1:250 

Validation WB

Non-supportive: Only bands not corresponding to the predicted size 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:30000 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). 
 

ANTIGEN VIEW

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CT47A6-001