HIST1H2AM

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA041189

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA041189 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

9 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

26 

Antigen sequence

AVRNDEELNKLLGGVTIAQGGVLPNI
 

Matching transcripts

HIST1H2AM-001 - ENSP00000352627 [92%]
 

Other gene match

H2AFY2 - ENSG00000099284 [81%]
H2AFY - ENSG00000113648 [85%]
HIST1H2AB - ENSG00000137259 [92%]
HIST1H2AA - ENSG00000164508 [96%]
HIST1H2AE - ENSG00000168274 [92%]
HIST1H2AC - ENSG00000180573 [92%]
HIST3H2A - ENSG00000181218 [92%]
HIST1H2AJ - ENSG00000182611 [92%]
HIST2H2AA3 - ENSG00000183558 [92%]
HIST2H2AC - ENSG00000184260 [92%]
HIST2H2AB - ENSG00000184270 [100%]
HIST1H2AK - ENSG00000184348 [92%]
HIST1H2AH - ENSG00000184825 [92%]
H2AFX - ENSG00000188486 [96%]
HIST1H2AI - ENSG00000196747 [92%]
HIST1H2AG - ENSG00000196787 [92%]
HIST1H2AD - ENSG00000196866 [92%]
HIST1H2AL - ENSG00000198374 [92%]
HIST2H2AA4 - ENSG00000203812 [92%]
H2AFJ - ENSG00000246705 [92%]
 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:500 

Validation IH

Supportive: The multi-targeting antibody yielding a staining pattern consistent with gene/protein characterization data for all of the genes 

         Western Blot

Image

 

Description

 

Target mass (kDa)

40.1, 39.6, 39.5, 39.2, 17.6, 15.1, 14.2, 14.1, 14.0, 13.9, 10.0 

Antibody dilution

1:250 

Validation WB

Non-supportive: Only bands not corresponding to the predicted size 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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HIST1H2AM-001