The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Weak to moderate cytoplasmic and occasional membranous positivity was observed in most of the normal tissues. Pancreatic islets, lung macrophages, breast and myocytes as well as basal cells in prostate, squamous and respiratory epithelium and showed strong staining. Cilia in bronchus and fallopian tubes were distinctly stained. The liver, alveolar cells, trophoblastic cells, glial cells and spleen were negative.
Immunohistochemical staining of human skin shows strong cytoplasmic positivity in stratum granulosum and stratum basale.
Summary
Weak to moderate cytoplasmic and occasional membranous positivity was observed in most of the normal tissues. Pancreatic islets, lung macrophages, breast and myocytes as well as basal cells in prostate, squamous and respiratory epithelium and showed strong staining. Cilia in bronchus and fallopian tubes were distinctly stained. The liver, alveolar cells, trophoblastic cells, glial cells and spleen were negative.
Validation
One antibody yielding a staining pattern and there is no available gene/protein characterization data or the staining pattern is partly consistent with or contradicted by limited gene/protein characterization data
