C2orf74

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA026826

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA026826 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

7 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

133 

Antigen sequence

DANGGVDCAAAKVVTSNPEDHERILMQVMNLNVPMRPGILVQRQSKEVLA
TPLENRRDMEAEEENQINEKQEPENAGETGQEEDDGLQKIHTSVTRTPSV
VESQKRPLKGVTFSREVIVVDLGNEYPTPRSYT
 

Matching transcripts

C2orf74-202 - ENSP00000402915 [100%]
C2orf74-201 - ENSP00000398725 [81%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in parietal cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:110 

Validation IH

Uncertain: One antibody yielding a staining pattern and there is no available gene/protein characterization data or the staining pattern is partly consistent with or contradicted by limited gene/protein characterization data 

         Immunofluorescence

Image

 

Description

Immunofluorescent staining of human cell line U-251 MG shows positivity in centrosome & nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

1:100 

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available. 

         Western Blot

Image

 

Description

 

Target mass (kDa)

21.9, 13.2 

Antibody dilution

1:250 

Validation WB

Non-supportive: Only bands not corresponding to the predicted size 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:12000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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C2orf74-201
 
C2orf74-202