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SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Plasma membrane, Cytoplasm

Additional location(s)

Centrosome 

Staining summary

Mainly localized to the plasma membrane, cytoplasm and nucleus.

Reliability (APE)

Medium

Antibodies in assay

HPA021165, HPA021753, HPA021760
 

Antibody HPA021165

 

Antibody HPA021753

 

Antibody HPA021760

 

         Cell line A-431

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Location(s)

Cytoplasm, Centrosome Nucleus, Plasma membrane, Cytoplasm Nucleus, Nucleoli, Cytoplasm 

Staining characteristics

   

Staining intensity

Weak Weak Weak 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Cytoplasm Nucleus, Cytoplasm   

Staining characteristics

    

Staining intensity

Weak Weak   

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
   

Comment

    

         Cell line U-251 MG

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Location(s)

Cytoplasm, Centrosome Nucleus, Cytoplasm Plasma membrane, Cytoplasm 

Staining characteristics

   

Staining intensity

Moderate Weak Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm & centrosome.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus, plasma membrane & cytoplasm.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in plasma membrane & cytoplasm.
 

Summary

Staining of cytoplasm in all three cell lines with additional staining of centrosomes in U-251 MG and A-431.   Staining of nucleus and cytoplasm in all three cell lines with additional staining of plasma membrane in A-431.   Staining of cytoplasm and plasma membrane in U-251 MG. Staining of nucleus, nucleoli and cytoplasm in A-431.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Centrosome
Cytoplasm
Nucleus
Plasma membrane