Fields »

SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane, Vesicles

Additional location(s)

 

Staining summary

Localized to plasma membrane and vesicles.

Reliability (APE)

Low

Antibodies in assay

CAB001809, HPA019527, HPA020912
 

Antibody CAB001809

 

Antibody HPA019527

 

Antibody HPA020912

 

         Cell line A-431

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Location(s)

 Cytoplasm, Vesicles Plasma membrane, Vesicles 

Staining characteristics

   

Staining intensity

Negative Strong Moderate 

Validation

No staining.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

   

         Cell line U-2 OS

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Location(s)

 Plasma membrane, Cytoplasm Plasma membrane, Vesicles 

Staining characteristics

 Granular  

Staining intensity

Negative Strong Moderate 

Validation

No staining.
 
Location not consistent with experimental gene/protein characterization data.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

   

         Cell line U-251 MG

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Location(s)

 Cytoplasm Plasma membrane, Vesicles 

Staining characteristics

   

Staining intensity

Negative Moderate Moderate 

Validation

No staining.
 
Location not consistent with experimental gene/protein characterization data.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows no positivity.
 

Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm & vesicles.
 

Immunofluorescent staining of human cell line A-431 shows positivity in plasma membrane & vesicles.
 

Summary

No staining was observed in any of the three cell lines.   Staining of cytoplasm in all three cell lines, with additional staining of vesicles in A-431 and plasma membrane in U-2 OS.   Staining of plasma membrane and vesicles in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Plasma membrane
Endosome