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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Centrosome

Additional location(s)

Cytoplasm 

Staining summary

Staining of centrosome and nucleus in all three cell lines. Additional staining of cytoplasm in A-431 and U-2 OS.

Reliability (Single)

Antibodies in assay

HPA014232
 

Antibody HPA014232

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Centrosome 

Staining characteristics

 

Staining intensity

Moderate 

Validation

The multi-targeting antibody yielding a staining pattern consistent with available gene/protein characterization data for at least one of the genes but not all.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Centrosome 

Staining characteristics

Granular 

Staining intensity

Moderate 

Validation

The multi-targeting antibody yielding a staining pattern consistent with available gene/protein characterization data for at least one of the genes but not all.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Centrosome 

Staining characteristics

 

Staining intensity

Weak 

Validation

The multi-targeting antibody yielding a staining pattern consistent with available gene/protein characterization data for at least one of the genes but not all.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli & centrosome.
 

Summary

Staining of centrosome and nucleus in all three cell lines. Additional staining of cytoplasm in A-431 and U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Centrosome
Cytoplasm
Nucleus but not nucleoli