The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human prostate shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human kidney shows distinct positivity in extracellular material.
Summary
A majority of normal tissues displayed weak to moderate cytoplasmic immunoreactivity. Strong positivity was observed in Leydig cells, renal tubules, trophoblastic cells of placenta and in few glandular epithelia.
Most normal cells showed weak to moderate cytoplasmic positivity. Plasma displayed moderate immunoreactivity.
Airway epithelium, gastric mucosa, endocrine glands and male genitalia showed moderate cytoplasmic positivity. The adrenal gland showed strong staining. Plasma was distinctly stained. Most other normal tissues were negative.
Validation
The multi-targeting antibody yielding a staining pattern consistent with gene/protein characterization data for at least one of the genes whereas any of the other genes has no available gene/protein characterization data or is contradicted by gene/protein
The multi-targeting antibody yielding a staining pattern consistent with gene/protein characterization data for at least one of the genes whereas any of the other genes has no available gene/protein characterization data or is contradicted by gene/protein
One antibody yielding a staining pattern which is consistent with gene/protein characterization data
