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SUBCELLULAR LOCATION

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Main location(s)

Nuclear membrane

Additional location(s)

Nucleus but not nucleoli, Mitochondria 

Staining summary

Staining of nucleus and nuclear membrane in all three cell lines with additional staining of mitochondria in A-431.

Reliability (Single)

Antibodies in assay

HPA036640
 

Antibody HPA036640

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Nuclear membrane, Mitochondria 

Staining characteristics

 

Staining intensity

Moderate 

Validation

The multi-targeting antibody yielding a staining pattern consistent with available gene/protein characterization data for at least one of the genes but not all.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Nuclear membrane 

Staining characteristics

 

Staining intensity

Strong 

Validation

The multi-targeting antibody yielding a staining pattern not consistent with available gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Nuclear membrane 

Staining characteristics

Spotty 

Staining intensity

Strong 

Validation

The multi-targeting antibody yielding a staining pattern not consistent with available gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli, nuclear membrane & mitochondria.
 

Summary

Staining of nucleus and nuclear membrane in all three cell lines with additional staining of mitochondria in A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Mitochondria
Nuclear membrane
Nucleus but not nucleoli