The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
High cytoplasmic and membranous expression in renal tubules, bile duct cells and pancreatic ducts. Caution: Based on antibodies targeting proteins from multiple genes.
Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in cells of tubules.
Immunohistochemical staining of human kidney shows strong luminal membrane and additional cytoplasmic positivity in renal tubules.
Summary
Strong cytoplasmic and membranous positivity was seen in cells of renal tubules, bile duct cells and pancreatic ducts. A subset of cells in gastro-intestinal tract and alveolar cells showed moderate staining. Endothelial cells were distinctly stained. The remaining normal tissues were generally negative.
Gall bladder, renal tubules, smooth muscle cells and subsets of cells in skin showed strong cytoplasmic and/or membranous positivity. Respiratory epithelium, fallopian tubes, endometrial and cervical glands were moderate stained. Remaining normal cells were weakly stained or negative.
Validation
The multi-targeting antibody yielding a staining pattern consistent with gene/protein characterization data for at least one of the genes whereas any of the other genes has no available gene/protein characterization data or is contradicted by gene/protein
The multi-targeting antibody yielding a staining pattern consistent with gene/protein characterization data for at least one of the genes whereas any of the other genes has no available gene/protein characterization data or is contradicted by gene/protein
