GOLGA8H

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA045397

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA045397 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

10 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

26 

Antigen sequence

KHGDLREVTLTSSAQGEAREDPLLDK
 

Matching transcripts

GOLGA8H-001 - ENSP00000456894 [92%]
 

Other gene match

GOLGA8I - ENSG00000153666 [92%]
GOLGA8Q - ENSG00000178115 [100%]
GOLGA8J - ENSG00000179938 [88%]
GOLGA8R - ENSG00000186399 [100%]
GOLGA8M - ENSG00000188626 [92%]
GOLGA8O - ENSG00000206127 [100%]
GOLGA8N - ENSG00000232653 [100%]
GOLGA8K - ENSG00000249931 [96%]
GOLGA8T - ENSG00000261247 [96%]
GOLGA8S - ENSG00000261739 [92%]
 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in paneth cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:150 

Validation IH

Uncertain: The multi-targeting antibody yielding a staining pattern partly consistent with limited gene/protein characterization data or there is no available gene/protein characterization data for all or at least one of the genes 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

71.8, 71.6, 71.5, 71.3, 70.4, 67.1, 50.5 

Antibody dilution

1:250 

Validation WB

Uncertain: No bands detected 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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GOLGA8H-001