CELL LINES

Name

Description of cell line

Original reference

Origin

Sex & Age 

Source

Myeloid

HEL Erythroleukemia cell line (AML M6 in relapse after treatment for Hodgkin's disease) Martin et al., Science 216: 1233 (1982) Peripheral blood Male, 30 DSMZ
HL-60 Acute promyelocytic leukemia (APL) cell line Collins et al., Nature 270: 347-349 (1977) Peripheral blood Female, 35 DSMZ
HMC-1 Mastcell leukemia cell line Butterfield et al., Leukemia Res. 12: 345-355 (1988) Peripheral blood Male, 52 KN1
K-562 Chronic myeloid leukemia (CML) cell line Lozzio et al., J Natl Cancer Inst 50: 535 (1973) Pleural effusion Female, 53 DSMZ
NB-4 Acute promyelocytic leukemia (APL) cell line Lanotte et al., Blood 77: 1080 (1991) Bone marrow Female, 23 DSMZ
THP-1 Acute monocytic leukemia (AML) cell line Tsuchiya et al., Int J Cancer 26: 171 (1980) Peripheral blood Male, 1 DSMZ
U-937 Myelomonocytic histiocytic lymphoma cell line Sundström C, Nilsson K., Int J Cancer 17:565-577 (1976) Pleural effusion Male, 37 KN1

Lymphoid

Daudi Human Burkitt lymphoma cell line Klein et al., Cancer Res 28: 1300 (1968) Orbital biopsy Male, 16 DSMZ
HDLM-2 Hodgkin lymphoma cell line Drexler et al., Leuk Res 10: 487 (1986) Pleural effusion Male, 74 DSMZ
Karpas-707 Multiple myeloma cell line Karpas et al., Lancet Apr 24;1(8278): 931-3 (1982) Bone marrow and peripheral blood Male, 53 KN1
LP-1 Multiple myeloma cell line Pegoraro et al., Blood 73: 1020 (1989) Peripheral blood Female, 56 DSMZ
MOLT-4 Acute lymphoblastic leukemia (T-ALL) cell line Minowada et al., J Natl Cancer Inst 49: 891 (1972) Peripheral blood Male, 19 DSMZ
REH* Pre-B cell leukemia cell line (ALL, first relapse) Rosenfeld et al., Nature 267: 841–3 (1977) Peripheral blood Female, 15 DSMZ
RPMI-8226 Multiple myeloma cell line Matsuoka et al., Proc Soc Exp Biol Med 125: 1246 (1967) Peripheral blood Male, 61 DSMZ
U-266/70 Multiple myeloma cell line (1970, IL-6-dependent) Nilsson et al Clin Exp Immunol 7:477-489 (1970) Peripheral blood Male, 53 KN1
U-266/84 Multiple myeloma cell line (1984, in vitro differentiated) Hellman et al., Eur J Immunol 18:905-910 (1988) Peripheral blood Male, 53 KN1
U-698 B-cell lymphoma cell line (lymphoblastic lymphosarcoma) Nilsson et al., Int J Cancer 13: 808-823 (1974) Tonsil Male, 7 DSMZ

Brain

SH-SY5Y Metastatic neuroblastoma, clonal subline of neuroepithelioma cell line SK-N-SH Biedler et al., Cancer Res 33: 2643 (1973) Bone marrow Female, 4 DSMZ
U-138 MG Glioblastoma cell line Ponten et al., Acta Pathol Microbiol Scand 74: 465 (1968) Brain Male, 47 DSMZ
U-251 MG Glioblastoma cell line Westermark B., Int J Cancer12: 438 (1973) Brain Male BW2
U-87 MG** Glioblastoma, astrocytoma cell line Ponten J., Macintyre EH., Acta Pathol. Microbiol. Scand. 74: 465-486 (1968) Brain LGC/ATCC

Lung

A-549 Lung carcinoma cell line Giard et al., J Natl Cancer Inst 51: 1417 (1973) Lung Male, 58 DSMZ
SCLC-21H Small cell lung carcinoma cell line Bepler et al., J Cancer Res Clin Oncol 113: 31 (1987) Pleural effusion Male, 46 DSMZ

Abdominal

CACO-2 Colon adenocarcinoma cell line Fogh J., et al. J. Natl. Cancer Inst. 58: 209 (1977) Colon Male, 72 DSMZ
CAPAN-2 Pancreas adenocarcinoma cell line Kyriazis et al., Cancer Res 46: 5810 (1986) Pancreas Male, 56 DSMZ
Hep-G2 Hepatocellular carcinoma cell line Aden et al., Nature 282: 615 (1979) Liver Male, 15 DSMZ

Breast, female reproductive system

AN3-CA Metastatic endometrial adenocarcinoma cell line Dawe et al., J Natl Cancer Inst 33: 441 (1964) Lymph node Female, 55 DSMZ
EFO-21 Metastatic ovarian serous cystadenocarcinoma cell line Simon et al., J Natl Cancer Inst 70: 839 (1983) Ascites Female, 56 DSMZ
HeLa Cervical epithelial adenocarcinoma cell line Scherer et al., J Exp Med 97: 695 (1953) Cervix Female, 31 DSMZ
MCF-7 Metastatic breast adenocarcinoma cell line Soule et al., J Natl Cancer Inst 51: 1409 (1973) Pleural effusion Female, 69 DSMZ
SiHa Cervical squamous cell carcinoma cell line, integrated 1-2 copies of HPV16 Friedl F. et al.Proc., Soc. Exp. Biol. Med. 135: 543-545 (1970) Cervix Female, 55 LGC/ATCC
SK-BR-3 Metastatic breast adenocarcinoma cell line Fogh J, editor. Human tumor cells in vitro. New York: Plenum Press (1975) Pleural effusion Female, 43 LGC/ATCC

Urinary, male reproductive system

NTERA-2 Metastatic embryonal carcinoma cell line, cloned from TERA-2 Fogh & Trempe, Human Tumor Cells in Vitro : 115 (1975) Male, 22 DSMZ
PC-3 Metastatic poorly differentiated prostate adenocarcinoma cell line Kaighn et al., Invest Urol 17: 16-23 (1979) Bone marrow Male, 62 DSMZ
RT-4 Urinary bladder transitional cell carcinoma cell line Rigby et al., Br J Cancer 24: 746 (1970) Urinary bladder Male, 63 DSMZ/ECACC

Skin

A-431 Epidermoid carcinoma cell line Giard et al., J Natl Cancer Inst 51: 1417 (1973) Skin Female, 85 DSMZ
HaCaT Keratinocyte cell line Boukamp et al., J Cell Biol Mar 106(3): 761 (1988) Skin Male NEF3
SK-MEL-30 Metastatic malignant melanoma cell line Carey et al., Proc Natl Acad Sci USA 73: 3278 (1976) Subcutis Male, 67 DSMZ
WM-115 Malignant melanoma cell line Westermark B. et al, Proc Natl Acad Sci U S A. Oct; 83(19): 7197 (1986) Skin Female, 58 BW2

Sarcoma

RH-30 Metastatic rhabdomyosarcoma cell line Douglass et al., Cytogenet Cell Genet 45: 148-155 (1987) Bone marrow Male, 17 DSMZ
U-2 OS Osteosarcoma cell line Ponten J., Saksela E. Int. J. Cancer 2: 434 (1967) Bone Female, 15 LGC/ATCC
U-2197 Malignant fibrous histiocytoma cell line Genberg et al., Am J Pathol 135: 1185 (1989) Subcutis Male, 79 DSMZ

Miscellaneous

BEWO Metastatic choriocarcinoma cell line Pattillo et al., Cancer Res 28: 1231-1236 (1968) Brain Male DSMZ
HEK 293 Embryonal kidney cell line, transformed by adenovirus type 5 Graham et al., J Gen Virol 36: 59 (1977) Embryonal kidney Female DSMZ
HTh 83 Anaplastic thyroid carcinoma cell line Dahlman et al., J. Pathol. 191: 376-386, 2000 Thyroid Male, 67 NEH4
TIME Telomerase-immortalized human microvascular endothelial cells (pooled) Venetsanakos et el., Exp Cell Res., Feb 1;273(1): 21-33 (2002) Skin Male LCW5

Mouse

NIH 3T3 Mouse embryonal fibroblast cell line Aaronson et al., J Cell Physio 72(2): 141-148 (1968) Embryo DSMZ

Notes

*
REH was previously listed as KM3. Due to misidentification, KM3 has been deleted from the DSMZ catalogue and the true identity is REH. DSMZ - False leukemia and lymphoma cell lines. Drexler et al., False leukemia-lymphoma cell lines: An update on over 500 cell lines. Leukemia 17: 416-426 (2003)
**
U-87 MG under investigation.

Source information

  1. Prof. Kenneth Nilsson, Uppsala University, Uppsala, Sweden
  2. Prof. Bengt Westermark, Uppsala University, Uppsala, Sweden
  3. Prof. Norbert E Fusenig, DKFZ, Heidelberg, Germany
  4. Assoc. Prof. Nils-Erik Heldin, Uppsala University, Uppsala, Sweden
  5. Prof. Martin McMahon, UCSF Comprehensive Cancer Center, San Fransisco, USA

Patient cell samples

Name

Description

Samples

Cells

PBMC Peripheral Blood Mononuclear Cells 15

Leukemia cells

AML Acute Myeloid Leukemia 4
B-ALL B-cell Acute Lymphoid Leukemia 3
B-CLL B-cell Chronic Lymphoid Leukemia 8
CML Chronic Myeloid Leukemia 5
T-ALL T-cell Acute lymphoid leukemia 2

Hematopoietic cell lines

Schematic description of hematopoietic differentiation lineages, and cellular origin for hematopoietic cell lines.