< No: 17 >

Knowledge-based portal

A milestone for the HPA effort was achieved with the inclusion of expression data for approximately 50% of human protein-coding genes. This was achieved in 2010 and an updated portal was launched. An important new feature was a cancer view. In addition, a new concept for subcellular localization of proteins using confocal microscopy was described.

Key publication

Other selected publications

  • Mathivanan S et al., Human Proteinpedia enables sharing of human protein data. Nat Biotechnol. (2008)
    PubMed: 18259167 DOI: 10.1038/nbt0208-164

  • Bourbeillon J et al., Minimum information about a protein affinity reagent (MIAPAR). Nat Biotechnol. (2010)
    PubMed: 20622827 DOI: 10.1038/nbt0710-650

  • M. Gry et al., Tissue-specific protein expression in human cells, tissues and organs. J Proteomics Bioinform (2010)
    DOI: 10.4172/jpb.1000153

  • Legrain P et al., The human proteome project: current state and future direction. Mol Cell Proteomics. (2011)
    PubMed: 21742803 DOI: 10.1074/mcp.M111.009993

  • Fagerberg L et al., Large-scale protein profiling in human cell lines using antibody-based proteomics. J Proteome Res. (2011)
    PubMed: 21726073 DOI: 10.1021/pr200259v

  • Colwill K et al., A roadmap to generate renewable protein binders to the human proteome. Nat Methods. (2011)
    PubMed: 21572409 DOI: 10.1038/nmeth.1607

Figure legend: The HPA summary view in 2010 for the human estrogen receptor 1 (ESR1), displaying expression data for the target protein in normal and cancer tissues. Adapted from M. Uhlén et al. (2010).

Key facts

  • The HPA contains information about 10,118 protein-coding genes in 44 tissues and organs
  • This corresponds to >50% of the 19,559 human entries as defined by the Ensembl genome browser
  • A new cancer view has been designed, with tumor tissues from 216 patients representing 20 cancers
  • A new feature is a section for subcellular localization of proteins
  • A new concept was introduced for validation of antibodies based on paired antibodies