The Fields button to the right of the Search area can be used to perform more specific and/or complex searches. When clicking the Fields button, three fields will appear under the original Search area. The leftmost field contains the logical operators AND and NOT. The middle part contains the Field field where you can restrict your search to for example only include proteins located on a certain chromosome, having a specific subcellular location, having antibodies validated in a particular assay, or having a certain tissue expression. Depending on your choice of Field term, one or more fields may appear to the right, allowing you to specify for example which tissue, cell type or level of expression you are interested in. After clicking Add you can either click Search to view the results of your query or click Fields to add more search terms to your query.
Below are some examples of different types of searches that can be performed.
Organ related searches
To find proteins at least moderately expressed in at least 25% of the tissues included in the female reproductive system, but not expressed in any of the tissues included in the male reproductive system:
Click Fields Select Organ expression (IH) in the Field field
Select Breast and female reproductive system (Female tissues) in the Organ field
Select > and 50% in the Fraction tissues fields
Select Moderate and Strong by clicking on both in the Expression field
Click Add and then click Fields The operator in the left field should be AND Select Organ expression (IH) in the Field field
Select Male reproductive system (Male tissues) in the Organ field
Select = and 100% in the Fraction tissues fields
Select Negative in the Expression field
Click Add Click Search
To find proteins expressed in CNS and not in hematopoietic cells:
Click Fields Select Organ expression (IH) in the Field field
Select Central nervous system (Brain) in the Organ field
Select > and 50% in the Fraction tissues fields
Select Strong in the Expression field
Click Add and then click Fields The operator in the left field should be AND Select Organ expression (IH) in the Field field
Select Blood and immune system (Hematopoietic) in the Organ field
Select = and 100% in the Fraction tissues fields
Select Negative in the Expression field
Click Add Click Search
To find proteins expressed in glomeruli but not in tubuli that are possibly involved in glomeruli function:
Click Fields Select Tissue expression (IH) in the Field field
Select Kidney in the Tissue field
Select Cells in tubules in the Cell type field
Select Negative in the Expression field
Click Add and then click Fields The operator in the left field should be AND Select Tissue expression (IH) in the Field field
Select Kidney in the Tissue field
Select Cells in glomeruli in the Cell type field
Select Strong in the Expression field
Click Add Click Search
To find proteins differentially expressed in breast cancer:
Click Fields Select Cancer expression (IH) in the Field field
Select Breast cancer in the Tissue field
Select > and 50% in the Fraction tissues fields
Select Strong in the Expression field
Click Add and then click Fields The operator in the left field should be AND Select Cancer expression (IH) in the Field field
Select Breast cancer in the Tissue field
Select > and 20% in the Fraction tissues fields
Select Negative in the Expression field
Click Add Click Search
Subcellular location and protein class related search
To find proteins of the enzyme subclass oxidoreductases located in the mitochondria:
Click Fields Select Subcellular location (IF) in the Field field
Select Mitochondria in the Location field
Click Add and then click Fields Select Protein class in the Field field
Select Enzymes in the Class field
Select Oxidoreductases in the Subclass field
Click Add Click Search
