THE HUMAN PROTEIN ATLAS BLOG

Antibody validation and autoimmunity profiling

2016-01-26
Cross-reactivity HuProt Microarray Multiple Sclerosis Validation

Exploration of high-density protein microarrays for antibody validation and autoimmunity profiling

Protein fragments are used within the Human Protein Atlas project for the generation of antibodies, these fragments have also been utilized for the validation of the antibodies on antigen microarrays.

All antibodies produced by the Human Protein Atlas have been validated for target recognition on antigen microarrays comprising of their target antigen and 383 other protein fragments. In total over 42.000 protein fragments have been arranged in sets of 384...Read more


From primary to malignant - what changes?

2016-01-19
Cancer Malignant transformation Model system Proteomics Transcriptomics

Three examples of differentially expressed proteins during malignant transformation in a four step fibroblast cell line model.

Majority of differentially expressed genes are down-regulated during malignant transformation in a four-stage model

From primary to malignant - what changes? Bridging transcriptomics and proteomics to reveal the molecular changes during malignant transformation in a four-step cancer cell line model

In order to find out more about the changes that occur when a primary cell is transformed into an aggressive cancer cell, integration of biological information has proven to be successful. By bridging transcriptomics and antibody based proteomics data we showed how the major changes during malignant transformation could be scrutinized...Read more


Investigating autoantibody reactivity in lungs

2016-01-12
Autoantibody Autoimmunity Microarray Sarcoidosis

A study of autoimmune targets in sarcoidosis revealed higher reactivity in patients compared to controls for four proteins in both bronchoalveolar lavage (BAL) and serum.

Proteomic Profiling Reveals Autoimmune Targets in Sarcoidosis

Sarcoidosis is an inflammatory lung disease with unknown cause. Previous research has shown that sarcoidosis patients have generally higher levels of antibodies compared to healthy controls but so far the role and protein targets of these antibodies are not well understood.

In this study we used protein microarrays to investigate autoantibody targets in bronchoalveolar lavage (BAL) fluid in the search for autoantigens associated to disease...Read more