The human protein atlas blog


The human skin proteome

2016-02-23
Proteome Skin Tissue Atlas

The human skin-specific genes defined by transcriptomics and antibody-based profiling

Normal skin is consists mainly of epidermal cells in different phases of de-differentiation (keratinization) organized in several distinct layers with different appearance, functions, and molecular signatures. To characterize the skin-specific genes and the landscape of gene and protein expression in normal human skin in different layers and stages of differentiation, scientists from the Human Protein Atlas project have combined immunohistochemistry-based protein profiling and genome-wide deep sequencing of mRNA to compare skin with other normal tissue types...Read more


The human prostate proteome

2016-02-09
Cancer Prostate Proteome Tissue Atlas

The definition of the prostate specific proteome provides a starting point for further studies exploring the molecular repertoire of the prostate.

Novel biomarkers for prostate cancer found while defining the prostate specific transcriptome and proteomeTo better understand the function of the prostate and diseases associated with it, it is important to explore the molecular constituents that make up the prostate gland.

In a recent study by scientists from the Human Protein Atlas project and colleagues both gene and protein expression profiles were investigated in prostate tissue and compared to 26 other human tissues. The aim was to identify potential prostate specific biomarkers for potential clinical use...Read more


Systematic antibody validation using siRNA

2016-02-02
Cell Atlas Gene editing Immunocytochemistry Immunofluorescence siRNA Subcell Atlas

Fig 1a. The protein HNRNPUL1 is localized to the nucleus by staining with antibody HPA046290 (green)

Systematic antibody validation with siRNA for the Human Protein Atlas

Antibodies are among the most frequently used tools for basic research and clinical assays. For antibodies used in therapy or diagnostics, there are well-defined and strict guidelines that must be complied with before approval for clinical assays. For research antibodies, such guidelines have not yet been developed, despite the importance of demonstrating that they are specific, selective, and yield reproducible results in the immunoassay for which they are to be used...Read more


Human Protein Atlas talks in February

2016-02-01
Presentation

  • February 4, 16:40 PM, Brussels, Belgium
  • Presenter: Tove Alm

    Presentation: The Human Protein Atlas: a flagship resource for understanding the whole human proteome

    ELIXIR Europe Conference Open Data in Action: Life Science Data Infrastructure for Innovation

  • February 7, 12:20 PM, Lorne, Australia
  • Presenter: Emma Lundberg

    Presentation: Closing plenary: Characterizing human organelle proteomes - Towards a complete Cell Atlas

    21st Annual Lorne Proteomics Symposium

    ..Read more