The human protein atlas blog


Autoantibody targets in narcolepsy

2016-05-31
Autoantibody Autoimmunity Proteomics Vaccine

Anna Häggmark

A new study by researchers within the Human Protein Atlas project was just published in Autoimmunity .

The researchers analyzed the presence of autoantibodies in patients with vaccine-associated narcolepsy. In an initial screening, IgG reactivity to approximately 10000 protein fragments was investigated, revealing a large heterogeneity in which autoantibodies that are present in different individuals.

Anna Häggmark is the first author of the paper...Read more


Image of the week - Vesicles with GFP

2016-05-29

Figure 1. Staining of RAB5C (green) with DNA (blue) in HeLa cells.

Welcome to another HPA image of the week! This week we take a look at vesicles and another type of data present in the HPA.

The protein stained in Fig 1. is an image of RAB5C. This protein is found in vesicles and specifically, in lysosomes of the cell. This sample shows HeLa human cervical adenocarcinoma cells.

These cells are actually transgenic and made to express green fluorescent protein on the RAB5C protein (RAB5Cgfp). This is a method we use to check the validity of our antibodies...Read more


On childhood asthma and protein profiles in plasma

2016-05-24
Antibody Suspension Bead Array Asthma Human Protein Atlas Plasma

Protein levels of CCL5, HPGDS and NPSR1 in plasma

In a recent publication in European Journal of Allergy and Clinical Immunology, researchers use an affinity proteomics approach to analyze plasma profiles of a 362 proteins in 154 children with persistent or intermittent asthma and controls. To this end, antibody suspension bead arrays developed within the Human Protein Atlas was used.

Asthma is a common chronic childhood disease with many different phenotypes that need to be identified. Asthma affects approximately 300 million people worldwide and is characterized by bronchial hyperresponsiveness and reversible expiratory airflow limitation...Read more


Image of the week - Focal Adhesions by Shiverwarp

2016-05-20
Citizen Science Focal Adhesions Image of the week Project Discovery Subcell Atlas

Staining of CORO2B (green) with tubules (red) and DNA (blue) in U-251 MG cells.

Welcome to another HPA image of the week! This week's image was brought to us by citizen scientists in Project Discovery, and specifically by Shiverwarp who found this image while playing Project Discovery in EVE online.

The protein stained in Fig 1. is an image of Coronin, actin binding protein, 2B (CORO2B) found in the focal adhesions and at the plasma membrane of the cell. This sample shows U-251 MG human glioblastoma astrocytoma (brain) cells.

Focal adhesions are transmembrane groups of protein that allow the cell to "grab" the surrounding environment...Read more


Site director with a fascination for patterns

2016-05-16
Human Protein Atlas Immunocytochemistry Interview Microarray Proteomics RNA-seq Tissue Atlas

Cecilia Lindskog, site director of the Tissue Atlas

Time has come for the second interview with a researcher within the Human Protein Atlas project. Today we meet Cecilia Lindskog, site director of the Tissue Atlas.

– I have a Master of Science in Biomedicine and a Doctor of Philosophy in pathology from the Faculty of Medicine, Uppsala University. I joined the Human Protein Atlas project in 2006, and also have industry experience in the biotechnology industry, from Oncomark Ltd, Dublin, Ireland.

Cecilia Lindskog´s main research interests have always been understanding the biology and functions of different organs, and the underlying mechanisms leading to cancer and other diseases...Read more