|
|
 |
 |
Hereby, the Swedish Human Proteome Atlas (HPA) program, funded by
the (non-profit) Knut and Alice Wallenberg Foundation, invites
submission of antibodies from both academic and commercial sources to
be included in the human protein atlas (www.proteinatlas.org). All
antibodies will be validated by the HPA-program by a standard
procedure and antibodies that are accepted will be use in the tissue-
profiling program to generate high-resolution immunohistochemistry
images representing a wide spectrum of normal tissues and cancer
types. During the pilot project phase, the validation will be done
free of charge. For each antibody, 576 spots of human tissue from 360
different individuals are immunostained and manually annotated.
Academic collaborators will have access to all biological data at
least 6 months before the images will be published as part of the
Human Protein Atlas. Commercial vendors will receive a direct web-
link from the antibody information page on the Protein Atlas to a web-
link decided by the antibody provider.
The collaborator is asked to fill-in the excel-sheet form with information, such as:
- Gene/protein Id of target (Ensembl Id)
- Description of target used for the antibody generation
- Description of type of antibody (pAb, mAb of recAb)
- Results from quality assurance (preferably a web-link but otherwise pdf-file)
The submissions are prioritized by the HPA Priority committee based on the extent of the validation and the presence or absence of the protein target in the HPA pipeline. For submissions that are approved, the collaborator will be asked to send in a vial (free of charge) of each antibody approved. A minimum amount of 50 µg or equivalent will be needed for each antibody.
Submissions could range from a single antibody to several hundreds of antibodies. Use one line in the excel sheet per antibody. Send the submission form (excel) to:
Use of the antibodies by the HPA program
Each antibody is tested according to HPA-standard quality assurance procedures, i.e., on Western blots and on a special designed tissue microarray (TMA) initially using two antibody-dilutions and heat epitope retrieval in citrate buffer. In selected cases, other pre-treatment, EDTA buffer or enzyme based antigen retrieval methods will be tried. The number of test rounds for each antibody is restricted to maximum three. Each antibody approved by HPA will subsequently be used for immunostaining of eight TMAs representing 576 tissue spots.
Information about the antigen
Information about the antigen is also requested in the submission form. It is preferable (but optional) to give the size of the antigen in number of amino acids. In the comment field one can also add actual peptide sequence and information about purity check, sequence verification etc (optional).
Throughput and success rates
We foresee that approximately 800 to 1000 external antibodies can be pursued annually.
Priority
Not more than two antibodies to the same target protein will normally be pursued. Priority will thus be given to new targets not present (or only represented by one antibody) in the Protein Atlas or in the HPA pipeline. Priority will be given to antibodies with well-documented and relevant quality assurance (see quality assurance).
Cost
The cost for the profiling (Western blot analysis, immunohistochemistry, scanning and annotation) will (during the pilot project) be covered by the HPA program (Wallenberg Foundation).
Release of data
Academic collaborators, can ask that the release of the data on the public Protein Atlas should be delayed further (beyond six months) if publication(s) based on the results from the protein profiling are being prepared. In this case, the release of the images can be delayed (pending decision by the HPA priority committee) normally not longer than one year.
home | call for antibodies - description
|
|
|
|
|
|
