The pigment cell-specific proteome
There are two types of pigment cells, found primarily in the skin epidermis and eyes. Pigment cells produce melanin which protects against UV radiation and gives the skin, hair and eyes its color. Pigment cells also play a role in the immune system and serve as a blood-retinal barrier.
The pigment cell transcriptome
The scRNA-seq-based pigment cell transcriptome can be analyzed with regard to specificity, illustrating the number of genes with elevated expression in each specific pigment cell type compared to other cell types (Table 1). Genes with an elevated expression are divided into three subcategories:
As shown in Table 1, 203 genes are elevated in melanocytes compared to other cell types. Melanocytes are mainly located in the basal layer of the epidermis and their primary role is to produce and deliver melanin-pigment to keratinocytes through dendritic processes. Genes with elevated expression in melanocytes include MLANA and DCT, which encode proteins involved in the melanin-synthesis pathways.
Retinal pigment cells
Retinal pigment cells is a type of pigment cell in the body that currently lack scRNA-seq data at Human Protein Atlas. They´re found in the eye and produce melanin that protects photoreceptor cells from UV-radiation and they are involved in the transport of nutrients and ions between photoreceptor cells and blood vessels in the choroid. The proteins bestrophin 1 (BEST1) and solute carrier family 16 member 8 (SLC16A8) both play a role in the transport of molecules and have elevated expression in the retina.
Pigment cell function
The two pigment cell types are melanocytes and retinal pigment cells. Melanocytes originate from neural crest cells, while retina pigment cells originate from the optic neuroepithelium. The main objective of melanocytes is to produce melanin, which protects the skin against UV-radiation. It is melanin produced by melanocytes in the uveal part of the eye that gives eyes their color. When melanin is exposed to UV-radiation from the sun, it becomes darker to protect the skin from further damage to the tissue. The amount of melanin produced by the melanocytes differs between individuals but the amount of melanocytes is the same. Lack of melanin production can lead to a disease called albinism, where the individual has no pigmentation at all and it will affect both eye and hair pigmentation. Moreover, melanocytes have immune cell qualities such as phagocytosis, antigen presentation capabilities and cytokine production.
Retinal pigment cells form retinal pigment epithelium in the back of the eye, adjacent to the retina. The retinal pigment epithelium works as a retinal-blood barrier and protects the retina. Similarly to melanocytes, retinal pigment cells produce pigment, however instead of protecting the skin, retinal pigment cells protect the retina from UV-radiation. Retinal pigment cells are in constant connection with the photoreceptor cells of the retina and support these cells in various ways. They buffer ions and water between photoreceptor cells and blood. Pigment cells also store 11-cis-retinal, a molecule essential for the transformation of light into visual signals in photoreceptor cells. They can quickly supply photoreceptor cells with 11-cis-retinal, when this molecule is depleted in photoreceptor cells.
The histology of organs that contain pigment cells, including interactive images, is described in the Protein Atlas Histology Dictionary.
Here, the protein-coding genes expressed in pigment cells are described and characterized, together with examples of immunohistochemically stained tissue sections that visualize corresponding protein expression patterns of genes with elevated expression in different pigment cell types.
The transcript profiling was based on publicly available genome-wide expression data from scRNA-seq experiments covering 25 tissues and peripheral blood mononuclear cells (PBMCs). All datasets (unfiltered read counts of cells) were clustered separately using louvain clustering, resulting in a total of 444 different cell type clusters. The clusters were then manually annotated based on a survey of known tissue and cell type-specific markers. The scRNA-seq data from each cluster of cells was aggregated to mean normalized protein-coding transcripts per million (nTPM) and the normalized expression value (nTPM) across all protein-coding genes. A specificity and distribution classification was performed to determine the number of genes elevated in these single cell types, and the number of genes detected in one, several or all cell types, respectively.
It should be noted that since the analysis was limited to datasets from 25 tissues and PBMC only, not all human cell types are represented. Furthermore, some cell types are present only in low amounts, or identified only in mixed cell clusters, which may affect the results and bias the cell type specificity.
Relevant links and publications
Uhlén M et al., Tissue-based map of the human proteome. Science (2015)