Immunofluorescent images of formaldehyde-fixed cell lines are shown. Three different organelle markers are displayed as different channels in the multicolor images - nucleus stained in blue, microtubules in red and ER in yellow. The various cell structures that are demonstrated are always shown in the green channel using an antibody found in the Human Protein Atlas. The antibody id is linked to the corresponding Cell Atlas protein page. By using the "toggle channels"-buttons, the different channels can be turned on and off. Most cell structures can be highlighted in the cell illustration by hovering over them with the exception of the aggresome. Cytoplasmic bodies are highlighted as cytosol; cytokinetic bridge, midbody, midbody ring and mitotic spindle are highlighted as microtubules, cell junctions are highlighted as plasma membrane and nucleus is highlighted as nucleoplasm.
Staining of cell junctions in human cell line MCF-7 (HPA063559)
Scale bar represents 10µm
Cell junctions occur between two cells or between a cell and the extracellular matrix. Depending on the cell type, they may have different functions: from transmission of stress to channel formation. Some cell junctions are critical for cell-to-cell communication as well as signal transduction.
Staining of cell junctions can only be observed when the cells are in direct contact. The staining pattern is very specific and is only seen at the actual site of cell-cell connections.