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Module 12: Plasma Profiling
Group leader: Dr Jochen M. Schwenk

Personnel: Kimi Drobin (research engineer), Ulrika Igel (PhD-student), Maja Neiman (PhD-student), Burcu Ayoglu (PhD-student) and Peter Nilsson (researcher).

Responsibility: (i) To implement HPA antibodies into an array-based workflow (ii) for an undirected multi-parallel protein profiling of human serum/plasma and (iii) to identify antibodies targeting potential biomarkers.

Description: An antibody suspension bead array is employed to profile biotinylated serum/plasma samples. Each month, purified and validated antibodies are immobilized on fluorescently colour-coded beads to create series of bead arrays composed of 384 antibodies. During this discovery phase, a microtiter plate based workflow facilitates to profile a multi-disease cohort built on 24 diseases and more than 550 specimens. With diseased and relevant control samples included, antibodies giving rise to disease associated protein profiles are identified and selected for subsequent investigations. During validation, the procedure is designed to focus on dedicated single-disease cohorts of an increased size and the utilization of samples that are independent of those analyzed during the discovery phase.

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