In an article in a special edition of Proteomics, on protein arrays researchers from the Human Protein Atlas describe the development of a novel assay concept, which combines the flexibility and multiplexing capacity of single-binder assays and the specificity and sensitivity aspects of dual-binder assays. They developed a multiplexed dual-binder assay procedure, which is based on a sequential protein capture.
– This assay is a great addition to the antibody-array toolbox of the Biobank Profiling Group, both to support related assay development activities and for biomarker discovery applications, says Burcu Ayoglu, first author of the study...Read more
Exploration of high-density protein microarrays for antibody validation and autoimmunity profiling
Protein fragments are used within the Human Protein Atlas project for the generation of antibodies, these fragments have also been utilized for the validation of the antibodies on antigen microarrays.
All antibodies produced by the Human Protein Atlas have been validated for target recognition on antigen microarrays comprising of their target antigen and 383 other protein fragments. In total over 42.000 protein fragments have been arranged in sets of 384...Read more
September 20, 2018 - September 21, 2018
On September 20th, at 10 am, Mathias Uhlén will give a talk on "Enhanced validation of antibodies for research applications". The meeting is held at Chancellors' Building at the University of Bath Claverton road campus.
September 30, 2018 - October 3, 2018
HPA Presence at HUPO 2018On Sunday Sept 30 (11am-12pm), Jochen Schwenk will be hosting a session on Antibody Validation.On Monday October 1, Cristina Al-Khalili Szigyarto will present "Antibodypedia - an open access resource of validated antibodies".On Wednesday October 3, Ragna Häussler will give a talk on "Elucidating changes in plasma protein profiles related to bariatric surgery: an IMI DIRECT study." At 10.30 am Emma Lundberg will present "Dissecting the spatiotemporal subcellular distribution of the human proteome".Peter Nilsson presents "Autoantigen Anoctamin 2 associates with increased risk for multiple sclerosis".At 14.30 pm Cecilia Lindskog Bergström will present "Integrated omics for spatial mapping of the human proteome - understanding the molecular repertoire in health and disease".