Exploration of high-density protein microarrays for antibody validation and autoimmunity profiling
Protein fragments are used within the Human Protein Atlas project for the generation of antibodies, these fragments have also been utilized for the validation of the antibodies on antigen microarrays.
All antibodies produced by the Human Protein Atlas have been validated for target recognition on antigen microarrays comprising of their target antigen and 383 other protein fragments. In total over 42.000 protein fragments have been arranged in sets of 384...Read more
Version 14 of The Human Protein Atlas includes a new type of validation of antibodies that are used for determining the subcellular localization of a protein.
A set of antibodies have been analyzed in transgenic cell lines expressing GFP-tagged target protein at near-endogenous levels to confirm that the antibodies are capable of binding the target protein. The approved antibodies are then used to determine the subcellular localization of endogenous protein in a selection of cell lines. A high validation score is assigned to those genes where the same location(s) are observed for both tagged protein and protein detected using labelled antibody in non-transfected cells...Read more
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
The Human Protein Atlas applies antibodies for a variety of applications to map protein expression in different tissues and also at the subcellular level. Within the subcellular protein atlas, immunofluorescence (IF) is used to uncover the localization of proteins to different organelles. To ensure an accurate localization of each and very protein, the antibodies have to be specific to their target protein...Read more
November 14, 2018 - November 14, 2018
Dr Charlotte Stadler is Plenary Keynote Speaker presenting "An image based subcellular map of the human proteome" at the The 6th biennial Pharmaceutical Flow Cytometry and Imaging meeting, Stevenage, UK.Overview
December 8, 2018 - December 12, 2018
The Human Protein Atlas will be exhibiting at the 2018 ASCB EMBO meeting in San Diego. In booth number #624 you will meet the Cell Atlas team Emma Lundberg, Christian Gnann, Lovisa Åkesson and Peter Thul. They will also present four posters. Preliminary programme