< No: 20 >
2014


Integration of RNA and protein profiles

A new version of the HPA was launched with integration of protein expression and transcriptomics data. 27 tissues and 33 cell lines were analyzed, and a new classification was introduced based on the transcriptomics profiles across all major tissues. A new version of the HPA was launched with information from 21,900 antibodies corresponding to approximately 16,600 human genes (80% of the human protein-coding genes).

Key publication

  • Fagerberg L et al., Analysis of the human tissue-specific expression by genome-wide integration of transcriptomics and antibody-based proteomics. Mol Cell Proteomics. (2014)
    PubMed: 24309898 DOI: 10.1074/mcp.M113.035600

Other selected publications

  • Djureinovic D et al., The human testis-specific proteome defined by transcriptomics and antibody-based profiling. Mol Hum Reprod. (2014)
    PubMed: 24598113 DOI: 10.1093/molehr/gau018

  • Kampf C et al., The human liver-specific proteome defined by transcriptomics and antibody-based profiling. FASEB J. (2014)
    PubMed: 24648543 DOI: 10.1096/fj.14-250555

  • Gremel G et al., The human gastrointestinal tract-specific transcriptome and proteome as defined by RNA sequencing and antibody-based profiling. J Gastroenterol. (2015)
    PubMed: 24789573 DOI: 10.1007/s00535-014-0958-7

  • Lindskog C et al., The lung-specific proteome defined by integration of transcriptomics and antibody-based profiling. FASEB J. (2014)
    PubMed: 25169055 DOI: 10.1096/fj.14-254862

  • Kampf C et al., Defining the human gallbladder proteome by transcriptomics and affinity proteomics. Proteomics. (2014)
    PubMed: 25175928 DOI: 10.1002/pmic.201400201



Figure legend: The human tissues and organs analyzed using transcriptomics and incorporated into the HPA 2014 update. Adapted from Fagerberg et al. (2014).


Key facts

  • Transcriptomics analysis across 27 organs and tissues were included in a new version of the portal
  • A new classification principle was introduced based on whole-body, tissue-specific protein expression
  • Data indicated that tissue-enriched genes constituted 12% of all genes (n = 2,473)
  • 8% of all genes were not detected in any of the tissues analyzed