Tissue methods

Multiplex tissue profiling - protein localization in single-cell types

The Tissue resource has implemented a novel protocol for studying protein localization in single-cell types, cell states and subcellular structures in human tissue samples, utilizing multiplex immunohistochemistry-based fluorescence imaging. With the help of single-cell transcriptomics and an iterative staining-stripping method, 8 antibody panels have been developed to profile 9 different tissues. In version 25.0, 1106 proteins have been analyzed using multiplex tissue profiling. More information about the generation of the multiplex tissue profiling data can be found on the Methods page.
In addition, a new dataset of highly multiplexed immunofluorescence images has been generated, including 46 different antibodies, plus nuclear marker. The images are available in the Dictionary section by selecting a specific tissue. More information about the generation of these images can be found on the Methods page.

Learn about:

• Spatial analysis at a resolution not obtained by regular immunohistochemistry
• Overlap in expression between uncharacterized proteins and specific cell type markers
• Protein localization in single-cell types, cell states and subcellular structures

Multiplex antibody panels

An interactive lollipop plot showing the number of proteins profiled with each antibody panel. Click on the hexagons to obtain the corresponding list of proteins.

Spatial localization based on multiplex tissue profiling

List presenting the number of proteins mainly located to different cell types/structures based on manual annotation of images stained with multiplex tissue profiling.