A high-throughput tissue profiling platform was set up to allow comprehensive immunohistochemistry-based analysis of protein expression patterns in normal human tissues, cancer tissue, and cell lines. Altogether, more than 700 individual microarray samples were immunohistochemically stained for each antibody. A web-based annotation system was developed to allow for evaluation and scoring of immunohistochemical staining patterns in tissues, and the manual analysis was performed by pathologists in Sweden and India. Protocols were standardized to enable a global, immunohistochemistry-based protein profiling of unprecedented magnitude.
Other selected publications
Andersson AC et al., Analysis of protein expression in cell microarrays: a tool for antibody-based proteomics. J Histochem Cytochem. (2006)
PubMed: 16957166 DOI: 10.1369/jhc.6A7001.2006
Strömberg S et al., A high-throughput strategy for protein profiling in cell microarrays using automated image analysis. Proteomics. (2007)
PubMed: 17549799 DOI: 10.1002/pmic.200700199
Kampf C et al., Production of tissue microarrays, immunohistochemistry staining and digitalization within the human protein atlas. J Vis Exp. (2012)
PubMed: 22688270 DOI: 10.3791/3620
Figure legend: Each antibody in the HPA program has been used to stain more than 700 tissues using the HPA microarray platform.
- The HPA microarrays include 44 tissue types covering all essential organs and the 20 major forms of cancer
- The HPA cell microarray has also been developed covering 68 human cell lines
- More than 10 million immunohistochemistry images have been generated and manually annotated