Cell lines


Name Description of cell line Original reference Origin Sex & Age  Source Cellosaurus (ExPaSy)

Brain

AF22 iPS derived Neuroepithelia stem (NES) cell line Falk et al., PLoS One 7(1) (2012) Skin Female, 36 NA Uncategorised
SH-SY5Y Metastatic neuroblastoma, clonal subline of neuroepithelioma cell line SK-N-SH Biedler et al., Cancer Res 33: 2643 (1973) Bone marrow Female, 4 DSMZ CVCL_0019
Cancer cell line
U-251 MG Glioblastoma cell line Westermark B., Int J Cancer12: 438 (1973) Brain Male BW1 CVCL_0021
Cancer cell line
U-138 MG Glioblastoma cell line Ponten et al., Acta Pathol Microbiol Scand 74: 465 (1968) Brain Male, 47 DSMZ CVCL_0020
Cancer cell line
U-87 MG* Glioblastoma, astrocytoma cell line Ponten J., Macintyre EH., Acta Pathol. Microbiol. Scand. 74: 465-486 (1968) Brain Male, 44 LGC/ATCC CVCL_0022
Cancer cell line

Myeloid

HEL Erythroleukemia cell line (AML M6 in relapse after treatment for Hodgkin's disease) Martin et al., Science 216: 1233 (1982) Peripheral blood Male, 30 DSMZ CVCL_0001
Cancer cell line
NB-4 Acute promyelocytic leukemia (APL) cell line Lanotte et al., Blood 77: 1080 (1991) Bone marrow Female, 23 DSMZ CVCL_0005
Cancer cell line
HAP1 Haploid karyotype cell line from Chronic myelogenous leukemia Carette et al., Nature 477(7364): 340-343 (2011) Leukemia Male Horizon Discovery CVCL_Y019
Cancer cell line
HL-60 Acute promyelocytic leukemia (APL) cell line Collins et al., Nature 270: 347-349 (1977) Peripheral blood Female, 35 DSMZ CVCL_0002
Cancer cell line
HMC-1 Mastcell leukemia cell line Butterfield et al., Leukemia Res. 12: 345-355 (1988) Peripheral blood Male, 52 KN2 CVCL_0003
Cancer cell line
K-562 Chronic myeloid leukemia (CML) cell line Lozzio et al., J Natl Cancer Inst 50: 535 (1973) Pleural effusion Female, 53 DSMZ CVCL_0004
Cancer cell line
THP-1 Acute monocytic leukemia (AML) cell line Tsuchiya et al., Int J Cancer 26: 171 (1980) Peripheral blood Male, 1 DSMZ CVCL_0006
Cancer cell line
U-937 Myelomonocytic histiocytic lymphoma cell line Sundström C, Nilsson K., Int J Cancer 17:565-577 (1976) Pleural effusion Male, 37 KN2 CVCL_0007
Cancer cell line

Lymphoid

REH** Pre-B cell leukemia cell line (ALL, first relapse) Rosenfeld et al., Nature 267: 841–3 (1977) Peripheral blood Female, 15 DSMZ CVCL_1650
Cancer cell line
Daudi Human Burkitt lymphoma cell line Klein et al., Cancer Res 28: 1300 (1968) Orbital biopsy Male, 16 DSMZ CVCL_0008
Cancer cell line
HDLM-2 Hodgkin lymphoma cell line Drexler et al., Leuk Res 10: 487 (1986) Pleural effusion Male, 74 DSMZ CVCL_0009
Cancer cell line
Karpas-707 Multiple myeloma cell line Karpas et al., Lancet Apr 24;1(8278): 931-3 (1982) Bone marrow and peripheral blood Male, 53 KN2 CVCL_0010
Cancer cell line
MOLT-4 Acute lymphoblastic leukemia (T-ALL) cell line Minowada et al., J Natl Cancer Inst 49: 891 (1972) Peripheral blood Male, 19 DSMZ CVCL_0013
Cancer cell line
RPMI-8226 Multiple myeloma cell line Matsuoka et al., Proc Soc Exp Biol Med 125: 1246 (1967) Peripheral blood Male, 61 DSMZ CVCL_0014
Cancer cell line
U-266/70 Multiple myeloma cell line (1970, IL-6-dependent) Nilsson et al Clin Exp Immunol 7:477-489 (1970) Peripheral blood Male, 53 KN2 CVCL_0015
Cancer cell line
U-266/84 Multiple myeloma cell line (1984, in vitro differentiated) Hellman et al., Eur J Immunol 18:905-910 (1988) Peripheral blood Male, 53 KN2 CVCL_0016
Cancer cell line
U-698 B-cell lymphoma cell line (lymphoblastic lymphosarcoma) Nilsson et al., Int J Cancer 13: 808-823 (1974) Tonsil Male, 7 DSMZ CVCL_0017
Cancer cell line

Lung

A549 Lung carcinoma cell line Giard et al., J Natl Cancer Inst 51: 1417 (1973) Lung Male, 58 DSMZ CVCL_0023
Cancer cell line
HBEC3-KT Immortalized human bronchial epithelial cell line Central lung bronchiole Female, 65 Evercyte CVCL_X491
Telomerase immortalized cell line
SCLC-21H Small cell lung carcinoma cell line Bepler et al., J Cancer Res Clin Oncol 113: 31 (1987) Pleural effusion Male, 46 DSMZ CVCL_0024
Cancer cell line

Abdominal

CACO-2 Colon adenocarcinoma cell line Fogh J., et al. J. Natl. Cancer Inst. 58: 209 (1977) Colon Male, 72 DSMZ CVCL_0025
Cancer cell line
Hep G2 Hepatocellular carcinoma cell line Aden et al., Nature 282: 615 (1979) Liver Male, 15 DSMZ CVCL_0027
Cancer cell line
CAPAN-2 Pancreas adenocarcinoma cell line Kyriazis et al., Cancer Res 46: 5810 (1986) Pancreas Male, 56 DSMZ CVCL_0026
Cancer cell line

Breast, female reproductive system

HeLa Cervical epithelial adenocarcinoma cell line Scherer et al., J Exp Med 97: 695 (1953) Cervix Female, 31 DSMZ CVCL_0030
Cancer cell line
MCF7 Metastatic breast adenocarcinoma cell line Soule et al., J Natl Cancer Inst 51: 1409 (1973) Pleural effusion Female, 69 DSMZ CVCL_0031
Cancer cell line
SiHa Cervical squamous cell carcinoma cell line, integrated 1-2 copies of HPV16 Friedl F. et al.Proc., Soc. Exp. Biol. Med. 135: 543-545 (1970) Cervix Female, 55 LGC/ATCC CVCL_0032
Cancer cell line
AN3-CA Metastatic endometrial adenocarcinoma cell line Dawe et al., J Natl Cancer Inst 33: 441 (1964) Lymph node Female, 55 DSMZ CVCL_0028
Cancer cell line
EFO-21 Metastatic ovarian serous cystadenocarcinoma cell line Simon et al., J Natl Cancer Inst 70: 839 (1983) Ascites Female, 56 DSMZ CVCL_0029
Cancer cell line
HTERT-HME1 Immortalized mammary epithelial cell line Mammary gland Female, 53 Evercyte CVCL_3383
Telomerase immortalized cell line
SK-BR-3 Metastatic breast adenocarcinoma cell line Fogh J, editor. Human tumor cells in vitro. New York: Plenum Press (1975) Pleural effusion Female, 43 LGC/ATCC CVCL_0033
Cancer cell line
T-47d Invasive ductal carcinoma Keydar et al., Eur J Cancer. May;15(5):659-70 (1979) Mammary gland; derived from metastatic site: pleural effusion Female, 54 ATCC CVCL_0553
Cancer cell line

Renal, urinary, male reproductive system

HEK 293 Embryonal kidney cell line, transformed by adenovirus type 5 Graham et al., J Gen Virol 36: 59 (1977) Embryonal kidney Female DSMZ CVCL_0045
Transformed cell line
PC-3 Metastatic poorly differentiated prostate adenocarcinoma cell line Kaighn et al., Invest Urol 17: 16-23 (1979) Bone marrow Male, 62 DSMZ CVCL_0035
Cancer cell line
RPTEC TERT1 Proximal tubular epithelial cell line Wieser et al., Am J Physiol Renal Physiol 295(5): 1365-1375 (2008) Human kidney cortex Male Evercyte CVCL_K278
Telomerase immortalized cell line
RT4 Urinary bladder transitional cell carcinoma cell line Rigby et al., Br J Cancer 24: 746 (1970) Urinary bladder Male, 63 DSMZ/ECACC CVCL_0036
Cancer cell line
NTERA-2 Metastatic embryonal carcinoma cell line, cloned from TERA-2 Fogh & Trempe, Human Tumor Cells in Vitro : 115 (1975) Male, 22 DSMZ CVCL_0034
Cancer cell line

Skin

A-431 Epidermoid carcinoma cell line Giard et al., J Natl Cancer Inst 51: 1417 (1973) Skin Female, 85 DSMZ CVCL_0037
Cancer cell line
HaCaT Keratinocyte cell line Boukamp et al., J Cell Biol Mar 106(3): 761 (1988) Skin Male NEF3 CVCL_0038
Spontaneously immortalized cell line
SK-MEL-30 Metastatic malignant melanoma cell line Carey et al., Proc Natl Acad Sci USA 73: 3278 (1976) Subcutis Male, 67 DSMZ CVCL_0039
Cancer cell line

Sarcoma

ASC TERT1 Adipose tissue-derived mesenchymal stem cell line Wolbank S. et al., Tissue Eng Part A: Jul;15(7):1843-54 (2009) Adipose tissue Female Evercyte CVCL_U602
Telomerase immortalized cell line
LHCN-M2 Adherent myoblast cell line Zhu et al., Aging Cell 6(4): 515-523 (2007) Satellite cells (from pectoralis major muscle) Male, 41 Evercyte CVCL_8890
Telomerase immortalized cell line
RH-30 Metastatic rhabdomyosarcoma cell line Douglass et al., Cytogenet Cell Genet 45: 148-155 (1987) Bone marrow Male, 17 DSMZ CVCL_0041
Cancer cell line
U-2 OS Osteosarcoma cell line Ponten J., Saksela E. Int. J. Cancer 2: 434 (1967) Bone Female, 15 LGC/ATCC CVCL_0042
Cancer cell line

Skin

WM-115 Malignant melanoma cell line Westermark B. et al, Proc Natl Acad Sci U S A. Oct; 83(19): 7197 (1986) Skin Female, 58 BW1 CVCL_0040
Cancer cell line

Sarcoma

ASC diff Differentiated imortalized adipose-derived mesenchymal stem cell line Wolbank S. et al., Tissue Eng Part A: Jul;15(7):1843-54 (2009) Adipose tissue Female Evercyte Uncategorised
U-2197 Malignant fibrous histiocytoma cell line Genberg et al., Am J Pathol 135: 1185 (1989) Subcutis Male, 79 DSMZ CVCL_0043
Cancer cell line

Fibroblast

BJ Adherent fibroblast cell line Bodnar AG., et al. Science 279: 349-352 (1998) Foreskin Male LGC/ATCC CVCL_3653
Finite cell line
BJ hTERT+ Adherent BJ fibroblast cell line immortalized with ectopic expression of hTERT Bodnar et al., Science 279(5349): 349-352 (1998) Foreskin Male NA CVCL_6573
Telomerase immortalized cell line
BJ hTERT+ SV40 Large T+ Adherent BJ fibroblast cell line immortalized with ectopic expression of hTERT, transformed with SV40 Large-T infection Bodnar et al., Science 279(5349): 349-352 (1998) Foreskin Male NA Uncategorised
BJ hTERT+ SV40 Large T+ RasG12V Adherent BJ fibroblast cell line immortalized with ectopic expression of hTERT, transformed with SV40 Large-T infection, made metastasizing with oncogenic H-Ras Bodnar et al., Science 279(5349): 349-352 (1998) Foreskin Male NA Uncategorised
FHDF/TERT166 Immortalized human foreskin fibroblast cell line Foreskin Male Evercyte CVCL_GZ73
Telomerase immortalized cell line
HBF TERT88 Adherent brain fibroblast cell line NA Brain Female Evercyte CVCL_9Q52
Telomerase immortalized cell line

Endothelial

HUVEC TERT2 Umbilical vein endothelial cell line NA Umbilical vein Female Evercyte CVCL_9Q53
Telomerase immortalized cell line
HTEC/SVTERT24-B Immortalized thymic epithelial cell line Thyroid Female Evercyte CVCL_JM27
Transformed cell line
TIME Telomerase-immortalized human microvascular endothelial cells (pooled) Venetsanakos et el., Exp Cell Res., Feb 1;273(1): 21-33 (2002) Skin Male LCW54 CVCL_0047
Telomerase immortalized cell line

Miscellaneous

HTCEpi Telomerase-immortalized corneal epithelial cell line Robertson et al., Invest Ophthalmol Vis Sci 46(2): 470-478 (2005) Cornea Male, 62 Evercyte CVCL_AQ44
Telomerase immortalized cell line
BEWO Metastatic choriocarcinoma cell line Pattillo et al., Cancer Res 28: 1231-1236 (1968) Brain Male DSMZ CVCL_0044
Cancer cell line
HHSteC Primary hepatic stellate cells Liver 3H Biomedical Uncategorised
HSkMC Primary skeletal muscle cells Trapezius and erector spinae muscles 3H Biomedical Uncategorised

Mouse

NIH 3T3 Mouse embryonal fibroblast cell line Aaronson et al., J Cell Physio 72(2): 141-148 (1968) Embryo DSMZ CVCL_0594
Spontaneously immortalized cell line

Notes

*
Allen M, Bjerke M, Edlund H, Nelander S and Westermark B: Origin of the U87MG glioma cell line: Good news and bad news. Science Translational Medicine 31 Aug 2016: Vol. 8, Issue 354, pp. 354. DOI: 10.1126/scitranslmed.aaf6853
**
REH was previously listed as KM3. Due to misidentification, KM3 has been deleted from the DSMZ catalogue and the true identity is REH. DSMZ - False leukemia and lymphoma cell lines. Drexler et al., False leukemia-lymphoma cell lines: An update on over 500 cell lines. Leukemia 17: 416-426 (2003)

Source information

  1. Prof. Bengt Westermark, Uppsala University, Uppsala, Sweden
  2. Prof. Kenneth Nilsson, Uppsala University, Uppsala, Sweden
  3. Prof. Norbert E Fusenig, DKFZ, Heidelberg, Germany
  4. Prof. Martin McMahon, UCSF Comprehensive Cancer Center, San Fransisco, USA

Hematopoietic cell lines


Schematic description of hematopoietic differentiation lineages, and cellular origin for hematopoietic cell lines.