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RNA expression in the single cell type clusters identified in this tissue visualized by a UMAP plot (top) and a bar chart (bottom).
- UMAP PLOT visualizes the cells in each cluster; where each dot corresponds to a cell. For each individual cell, mouseover reveals read count and which cluster the cell belongs to. Hovering over a cluster name will highlight the corresponding cluster in the bar chart below. There are 2 options for color schemes: 1) cell type color, which is based on cell type groups used in the Single cell type part of the Single cell resource and 2) cluster color, which assigns a unique color to each cluster.
The cells are colored according to % of max (log2(read_count+1)/log2(max(read_count)+1)*100) in five different bins (<1%,<25%,<50%,<75%,≥75%)
- The LEGEND shows RNA expression (nTPM) in each cell type cluster. Hovering over the cluster name reveals nTPM value and number of included cells. Hovering over a bar highlights the corresponding cluster in the UMAP plot above. Color-coding can be toggled on the top of the page, between 2 options: 1) cell type color, which is based on cell type groups used in the Single cell type part of the Single cell resource and 2) cluster color, which assigns a unique color to each cluster.
Cell type color
Cluster color
Colored according to cell type group
Colored according to clusters
Read count filter:0
Cluster filter:
Cluster
Cell type
Expression (nCPM)
c-0
Cardiomyocytes
c-1
Cardiomyocytes
c-2
Cardiomyocytes
c-3
Cardiomyocytes
c-4
Fibroblasts
c-5
Cardiomyocytes
c-6
Macrophages
c-7
Endocardium
c-8
Pericytes
c-9
Cardiomyocytes
c-10
Cardiomyocytes
c-11
Epicardium
c-12
Cardiomyocytes
c-13
Vascular smooth muscle cell
c-14
Adipocytes
c-15
T-cells & Fibroblasts
c-16
Mast cells
c-17
Pericytes CD44+
CELL TYPE MARKERSi
The heatmap in this section shows expression of the currently selected gene (on top) and well-known cell type markers in the different single cell type clusters of this tissue. The panel on the left shows which cell type each marker is associated with. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Hover the mouse-pointer over the individual data points (squares) to see nTPM level and Z-score. Clicking on a gene name redirects to the corresponding gene page.
Z-score is when you normalize a variable such that the standard deviation is 1 and the mean is 0. Thus, all the genes are easier to compare, as they have the same center and distribution.
SINGLE CELL TYPE
Max-norm
Z-score
Fraction of highest expression (≥1 nTPM)
log2(nTPM+1) z-score
DVP - HEART MUSCLEi
Relative protein amount measured using Deep Visual Proteomics. Here, on the detail page, all the biological replicates are shown, compared to the median value displayed on the gene summary page. The matched, selected single cell type clusters for the RNA-Protein comparison is also shown. The selected Cell Type clusters have the same name and ID as in the original dataset, above.
Below, we show a thumbnail overview of the tissue section used for the DVP, as well as smaller cropped area, that is clickable for a high-resolution zoomable image, where you can switch on and off the segmentation mask that was used for sample collection.
Learn more about the DVP method and different markers used the cell types here
Immunofluorescence image with DVP segmentation contours, high resolution cropped area with cell type markers (More info). This is heart muscle, and includes TNNT2 labeling of cardiomyocytes as well as lectin (WGA) staining of cell membranes.
Cell type
Capillaries
RNA
Cluster
Cell type
Expression (nCPM)
c-7
Endocardium
c-8
Pericytes
c-13
Vascular smooth muscle cell
Protein
Sample name
Intensity
Capillaries_01
Capillaries_02
Capillaries_03
Capillaries_04
Capillaries_05
Immunofluorescence image with DVP segmentation contours, high resolution cropped area with cell type markers (More info). This is heart muscle, and inlcudes CD34 labeling of endothelial cells.
The Human Protein Atlas project is funded
