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Solid-phase sequencing

The principle of solid-phase DNA sequencing was first described in 1988 based on binding of biotinylated DNA to streptavidin-coated magnetic beads and selective elution of one strand using alkali. Solid-phase methods are now frequently integrated into many nextgeneration DNA sequencing methods as well as numerous molecular diagnostics applications.

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Figure legend: The principle of solid-phase sequencing using the biotin-streptavidin system. Adapted from M. Uhlén et al., (1992).

Key facts

  • Solid-phase DNA sequencing using the biotin-streptavidin interaction was described in 1988
  • Solid-phase methods are now used in the majority of next-generation sequencing methods
  • A search for “biotin streptavidin” in Google Scholar yields more than 40,000 publications