The core mast cell enriched transcriptome
Mast cells are a type of leucocyte responsible for inflammatory reactions, and have granules that contain a collection of pro-inflammatory substances, including histamine, heparin, chondroitin sulfate, peroxidase and platelet-activating factor, which are released upon detection of proinflammatory stimuli.
In this section we detail the predicted core mast cell transcriptome, highlighting genes classified as having predicted specificity in mast cells in multiple tissue types. Such genes could be useful general markers for mast cells across tissue beds, and are likely critical for mast cell specific functions. These genes are sub-divided into 3 categories, based on the number of tissues in which they were independently classified as mast cell enriched.
Protein expression of genes enriched in mast cells across tissue types
Carboxypeptidase A3 (CPA3) is classified as having specificity to mast cells across multiple tissue beds. This preproprotein is processed to generate a mature protease released by mast cells. Expression of this gene may be elevated in human asthma patients.
Another gene classified as having specificity to mast cells across multiple tissue beds include KIT Proto-Oncogene, Receptor Tyrosine Kinase KIT, a cytokine receptor through which signaling plays role in cell survival, proliferation, and differentiation.
Genes with predicted specificity in mast cells in individual tissues
A summary table of all genes with predicted high enrichment in mast cells in profiled tissues is provided below. Identified genes are subdivided into 3 categories in each tissue, based on the difference between the enrichment score in mast cells, compared to the other cell types profiled (see Methods Summary page for details):