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Antibody validation

The International Working Group for Antibody Validation (IWGAV) was formed to develop approaches for validating antibodies used in common research applications and to provide guidelines that ensure antibody reproducibility. The working group recommended five conceptual “pillars” for antibody validation to be used in an application-specific manner.

Key publication

Other selected publications

Figure legend: Proposed conceptual pillars for validation of antibodies. Adapted from Uhlén et al. (2016).

Key facts

  • A proposal for validation of antibodies has been suggested by IWGAV
  • Many antibody providers are now using principles from the IWGAV guidelines for antibody validation
  • Antibodypedia has adopted the IWGAV guidelines to improve evidence-based selection of antibodies
  • More than 10,000 antibodies in the HPA have been validated using the IWGAV pillars