The International Working Group for Antibody Validation (IWGAV) was formed to develop approaches for validating antibodies used in common research applications and to provide guidelines that ensure antibody reproducibility. The working group recommended five conceptual “pillars” for antibody validation to be used in an application-specific manner.

Key publication

• Uhlen M et al., A proposal for validation of antibodies. Nat Methods. (2016)
  PubMed: 27595404 DOI: 10.1038/nmeth.3995

Other selected publications

• Edfors F et al., Enhanced validation of antibodies for research applications. Nat Commun. (2018)
  PubMed: 30297845 DOI: 10.1038/s41467-018-06642-y

• Sikorski K et al., A high-throughput pipeline for validation of antibodies. Nat Methods. (2018)
  PubMed: 30377371 DOI: 10.1038/s41592-018-0179-8