Brain tissue expression of WNK1 in pons

PONS - Expression summary

Protein expressionⁱ

No data

RNA expressionⁱ

HPA Brain:	551.3	nTPM
FANTOM5:	300.6	Scaled Tags Per Million

Macaque:	179.1	nTPM
Mouse:	96.4	nTPM
Rat:	80.2	nTPM
Pig:	113.8	nTPM

PONS - SINGLE NUCLEI RNA EXPRESSIONⁱ RNA expression in the single nuclei cluster types identified in this brain region visualized by a UMAP plot (top) and a bar chart (to the right). - UMAP PLOT visualizes the cells in each cluster; where each dot corresponds to a cell. For each individual cell, mouseover reveals read count and which cluster the cell belongs to. Hovering over a cluster name will highlight the corresponding cluster in the bar chart two the right. There are 2 options for color schemes: 1) cell type color, which is based on cell type groups used in the Single cell type part of the Single cell resource and 2) cluster color, which assigns a unique color to each cluster. There are 3 options for cell intensities: 1) Intensity, which color the individual cells according to % of max (log2(read_count+1)/log2(max(read_count)+1)100) in five different bins (<1%,<25%,<50%,<75%,≥75%) 2) Interval, which color the individual cells according to fixed interval of read count (0,1,2-4,5-9,>10) 3) Hexagon, which color the hexagon on average read count of all cells inside the hexagon - The BAR CHART* shows RNA expression (nTPM) in each cluster type. Hovering over the cluster name reveals nTPM value and number of included cells. Hovering over a bar highlights the corresponding cluster in the UMAP plot above. Color-coding can be toggled on the top of the page, between 2 options: 1) cell type color, which is based on cell type groups used in the Single cell type part of the Single cell resource and 2) cluster color, which assigns a unique color to each cluster.

SINGLE NUCLEI BRAIN - PONS, NEURONSⁱ

Cell type color

Cluster color

Colored according to cell type group

Read count filter: 0

Cluster		Cell type
	c-1	Excitatory (VGLUT1/2)
	c-2	Excitatory (VGLUT1) ETV1+ Z...
	c-3	Excitatory (VGLUT1/2)
	c-4	Excitatory (VGLUT1) ETV1+ Z...
	c-5	Excitatory (VGLUT1/2)
	c-6	Excitatory (VGLUT1/2)
	c-7	Excitatory (VGLUT1/2)
	c-8	Inhibitory (GAD1/2) SLC6A5+
	c-9	Excitatory (VGLUT2)
	c-10	Excitatory (VGLUT1/2)
	c-11	Excitatory (VGLUT1/2)
	c-12	Unassigned
	c-13	Inhibitory (GAD1/2) RORB+ K
	c-14	Inhibitory (GAD1/2) SST+
	c-15	Excitatory (VGLUT1) ETV1+ Z...
	c-16	Excitatory (VGLUT2)
	c-17	Excitatory (VGLUT1/2)
	c-18	Inhibitory (GAD1/2)
	c-19	Inhibitory (GAD1) PENK+ SST
	c-20	Excitatory (VGLUT2) FOXP2+
	c-21	Excitatory (VGLUT2) LMX1A+
	c-22	Excitatory (VGLUT2) MEIS2+
	c-23	Inhibitory (GAD1/2) SLC6A5+...
	c-24	Inhibitory (GAD1/2) SLC6A5+...
	c-25	Serotonergic SLC6A4+ SLC18A...
	c-26	Inhibitory (GAD1/2) PVALB+ ...
	c-27	Excitatory (VGLUT2)
	c-28	(VGLUT3) PENK+
	c-29	MEIS2+
	c-30	LMX1A+
	c-31	Inhibitory (GAD1/2) TAC1+
	c-32	Excitatory (VGLUT2) PVALB+
	c-33	Inhibitory (GAD1/2) PVALB+ ...
	c-34	Inhibitory (GAD1/2)
	c-35	Excitatory (VGLUT2) SST+ ME...
	c-36	LC Noradrenergic SLC6A2+ SL...
	c-37	Excitatory (VGLUT2) CRH+ FO...
	c-38	Inhibitory (GAD1/2) PVALB+ ...
	c-39	Excitatory (VGLUT2)
	c-40	Excitatory (VGLUT2) PVALB+
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This data was generated by Siletti K et al. (2023), re-processed by HPA (see methods) and simplified for global overview

SINGLE NUCLEI BRAIN - PONS, NON-NEURONALⁱ

Cell type color

Cluster color

Colored according to cell type group

Read count filter: 0

Cluster		Cell type
	c-1	Oligodendrocytes
	c-2	Oligodendrocytes
	c-3	Oligodendrocyte progenitor ...
	c-4	Oligodendrocytes
	c-5	Oligodendrocytes
	c-6	Oligodendrocytes
	c-7	Microglia
	c-8	Astrocytes
	c-9	Oligodendrocytes
	c-10	Astrocytes (CD44+)
	c-11	Oligodendrocytes
	c-12	Fibroblasts
	c-13	Vascular endothelial cells
	c-14	Pericytes
	c-15	Ependymal cells
	c-16	Lymphocytes
	c-17	Macrophages
	c-18	Microglia (SPP1+ HIF1A+)
	c-19	Bergmann glia
	c-20	Immature oligodendrocytes
	c-21	Oligodendrocyte progenitor ...
	c-22	Vascular smooth muscle cell
	c-23	Monocytes
	c-24	Immature oligodendrocytes
	c-25	Fibroblasts (BNC2+ KCNMA1+ ...
	c-26	Choroid plexus epithelial c...
	c-27	Macrophage Microglia double
	c-28	OPC & lymphocytes doublets
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This data was generated by Siletti K et al. (2023), re-processed by HPA (see methods) and simplified for global overview

PONS - HPA Brain Stereo-seq

PONS - HPA Brain RNA-seq

Max subtype nTPMⁱ Max subtype nTPM is the value for the subtype with the largest average nTPM across the subtype samples.	551.3

Sample id

Sample description

nTPM

Anterior cochlear nucleus, ventral
nTPM: 551.3

2301	Female, age 85, both hemispheres	675.3
2188	Female, age 91, both hemispheres	654.9
2090	Male, age 80, both hemispheres	497.3
1984	Male, age 76, both hemispheres	377.7

Dorsal cochlear nucleus
nTPM: 470.3

2187	Female, age 91, both hemispheres	531.4
2802	Male, age 74, right hemisphere	493.4
2378	Male, age 80, both hemispheres	476.1
2370	Male, age 61, both hemispheres	430.5
2058	Male, age 76, both hemispheres	420.1

Dorsal tegmental nucleus
nTPM: 472.4

2083	Male, age 80, both hemispheres	505.8
2326	Male, age 74, both hemispheres	490.3
2358	Male, age 61, both hemispheres	469.5
1936	Male, age 76, left hemisphere	424.3

Dorsolateral tegmental area
nTPM: 455.8

2337	Male, age 61, both hemispheres	605.7
2654	Male, age 80, both hemispheres	509.4
2751	Female, age 85, both hemispheres	453.5
2141	Male, age 76, both hemispheres	368.1
2360	Male, age 74, both hemispheres	342.2

Kolliker-Fuse nucleus
nTPM: 521.4

2793	Female, age 91, left hemisphere	650.3
2163	Male, age 80, both hemispheres	485.4
2315	Male, age 76, right hemisphere	428.5

Lateral lemniscal nuclei
nTPM: 458.4

2320	Female, age 91, left hemisphere	557.2
2036	Male, age 80, both hemispheres	466.1
2169	Male, age 76, both hemispheres	451.6
2746	Female, age 85, left hemisphere	408.8
2453	Male, age 74, right hemisphere	408.5

Lateral parabrachial nucleus
nTPM: 478.1

2792	Male, age 76, both hemispheres	582.3
2790	Male, age 61, both hemispheres	484.7
2013	Male, age 80, both hemispheres	447.2
2139	Female, age 85, right hemisphere	398.0

Lateral vestibular nucleus
nTPM: 547.0

2311	Male, age 80, both hemispheres	577.9
2184	Male, age 76, both hemispheres	516.0

Locus coeruleus
nTPM: 353.0

2720	Male, age 74, both hemispheres	420.7
2162	Male, age 80, both hemispheres	418.7
2317	Female, age 72, unknown	350.9
2014	Male, age 55, unknown	341.0
1980	Male, age 76, both hemispheres	329.2
2123	Female, age 91, both hemispheres	328.8
2788	Male, age 74, unknown	281.8

Medial parabrachial nucleus
nTPM: 522.0

2170	Female, age 91, both hemispheres	597.8
2156	Male, age 61, both hemispheres	576.1
2463	Female, age 85, right hemisphere	497.2
2854	Male, age 80, both hemispheres	485.2
2786	Male, age 76, both hemispheres	453.8

Medial periolivary nuclei
nTPM: 425.5

2458	Female, age 91, both hemispheres	508.0
2081	Male, age 80, both hemispheres	494.6
2171	Female, age 85, both hemispheres	393.7
2063	Male, age 76, both hemispheres	392.5
2791	Male, age 74, right hemisphere	338.6

Motor facial nucleus
nTPM: 455.5

2697	Female, age 85, both hemispheres	541.7
2126	Male, age 61, both hemispheres	489.5
2795	Female, age 91, both hemispheres	464.6
2327	Male, age 80, both hemispheres	438.4
2365	Male, age 74, right hemisphere	408.8
2062	Male, age 76, both hemispheres	389.9

Motor trigeminal nucleus
nTPM: 491.0

2321	Female, age 91, both hemispheres	581.4
2335	Male, age 61, both hemispheres	488.8
2309	Male, age 76, both hemispheres	479.9
2560	Male, age 74, both hemispheres	453.9
2079	Male, age 80, both hemispheres	450.9

Nuclei of the trapezoid body
nTPM: 422.3

2166	Male, age 80, both hemispheres	506.5
2368	Female, age 91, both hemispheres	486.9
2159	Male, age 61, both hemispheres	411.0
1983	Male, age 76, both hemispheres	374.9
2735	Female, age 85, both hemispheres	332.1

Nucleus raphe magnus
nTPM: 415.3

2459	Female, age 91, both hemispheres	480.8
2328	Male, age 80, both hemispheres	469.7
2051	Male, age 76, both hemispheres	464.3
2455	Male, age 74, both hemispheres	392.2
2172	Female, age 85, both hemispheres	348.4
2160	Male, age 61, both hemispheres	336.1

Paramedian reticular nucleus
nTPM: 415.8

2052	Male, age 76, both hemispheres	448.0
2057	Male, age 80, both hemispheres	442.5
2050	Female, age 85, both hemispheres	389.3
2313	Male, age 61, both hemispheres	383.3

Pedunculopontine tegmental nucleus
nTPM: 421.3

2338	Male, age 61, both hemispheres	484.8
2334	Male, age 80, both hemispheres	445.5
1921	Female, age 85, both hemispheres	333.6

Pontine nuclei
nTPM: 433.2

2319	Female, age 91, both hemispheres	536.0
2115	Male, age 80, left hemisphere	510.1
2109	Female, age 72, unknown	459.8
1968	Male, age 76, left hemisphere	452.3
2454	Male, age 74, left hemisphere	435.5
1863	Male, age 55, unknown	429.8
2787	Female, age 85, both hemispheres	372.9
2727	Male, age 61, right hemisphere	269.2

Pontine raphe nucleus
nTPM: 355.9

2853	Male, age 76, midline	374.9
2122	Female, age 85, midline	336.9

Posteroventral cochlear nucleus
nTPM: 517.1

2135	Female, age 91, both hemispheres	673.6
2341	Male, age 80, both hemispheres	594.5
2371	Male, age 61, both hemispheres	476.4
2070	Male, age 76, both hemispheres	458.4
2752	Female, age 85, both hemispheres	382.6

Principal sensory trigeminal nucleus
nTPM: 446.4

2324	Male, age 76, both hemispheres	529.1
2367	Female, age 91, left hemisphere	519.6
2158	Male, age 61, both hemispheres	397.9
2008	Male, age 80, left hemisphere	339.0

Reticular pontine nucleus, caudal
nTPM: 483.0

2457	Female, age 91, both hemispheres	544.9
2559	Male, age 74, both hemispheres	494.1
2092	Male, age 76, both hemispheres	471.5
2332	Male, age 80, right hemisphere	421.5

Reticular pontine nucleus, oral
nTPM: 470.9

2366	Female, age 91, both hemispheres	581.9
2361	Male, age 74, both hemispheres	478.5
2167	Male, age 76, both hemispheres	465.5
1939	Male, age 80, both hemispheres	434.7
2012	Female, age 85, both hemispheres	393.9

Reticulotegmental nucleus
nTPM: 486.3

2322	Female, age 91, both hemispheres	523.1
2452	Male, age 74, both hemispheres	517.3
2333	Male, age 80, both hemispheres	484.5
2325	Male, age 76, both hemispheres	420.0

Spinal trigeminal nucleus, oral
nTPM: 420.1

2164	Male, age 80, both hemispheres	524.5
1978	Male, age 76, both hemispheres	405.4
2737	Female, age 85, both hemispheres	330.3

Subcoeruleus area
nTPM: 443.8

2369	Female, age 85, left hemisphere	508.3
2451	Male, age 74, both hemispheres	494.4
2089	Male, age 76, left hemisphere	460.4
1958	Male, age 80, both hemispheres	402.7
2323	Female, age 91, left hemisphere	353.1

Superior olive
nTPM: 452.2

2561	Female, age 91, both hemispheres	538.3
2020	Male, age 76, both hemispheres	498.6
2329	Male, age 80, right hemisphere	493.1
2364	Male, age 74, right hemisphere	382.3
1875	Female, age 85, both hemispheres	348.7

Ventral periolivary nuclei
nTPM: 434.3

2023	Male, age 76, both hemispheres	468.0
2794	Female, age 91, left hemisphere	446.3
2165	Male, age 80, both hemispheres	441.5
2130	Female, age 85, both hemispheres	381.6

Ventrolateral tegmental area, A5 NE cell group
nTPM: 439.5

2456	Female, age 91, both hemispheres	496.3
2161	Male, age 80, both hemispheres	470.9
2840	Female, age 85, both hemispheres	441.7
1959	Male, age 76, both hemispheres	425.3
2359	Male, age 61, both hemispheres	411.2
2449	Male, age 74, both hemispheres	391.6

PONS - FANTOM5 CAGEⁱ

Max subtype Scaled Tags Per Millionⁱ Max subtype Scaled Tags Per Million is the value for the subtype with the largest average Scaled Tags Per Million across the subtype samples.	300.6

FANTOM5 sample id

Sample description

Scaled Tags Per Million

Locus coeruleus
Scaled Tags Per Million: 300.6

FF:10182-103D2	60 years, female	378.5
FF:10165-103B3	76 years, female	222.8

Pons
Scaled Tags Per Million: 165.1

FF:10033-101E6

18-54 years, mixed

165.1

PONS - Macaque RNA-seq

Max subtype nTPMⁱ Max subtype nTPM is the value for the subtype with the largest average nTPM across the subtype samples.	179.1

Macaque sample

Sample description

nTPM

Pons
nTPM: 179.1

Samples: 2

Max nTPM: 197.7
Min nTPM: 160.5

Sample A		197.7
Sample B		160.5

PONS - Pig RNA-seqⁱ

Max subtype nTPMⁱ Max subtype nTPM is the value for the subtype with the largest average nTPM across the subtype samples.	113.8

Pig sample

Sample description

nTPM

Pons
nTPM: 113.8

Samples: 3

Max nTPM: 116.9
Min nTPM: 111.9

Sample A	Female	116.9
Sample B	Female	112.5
Sample C	Male	111.9

PONS - Mouse RNA-seqⁱ

Max subtype nTPMⁱ Max subtype nTPM is the value for the subtype with the largest average nTPM across the subtype samples.	96.4

Mouse sample

Sample description

nTPM

Pons
nTPM: 96.4

Samples: 4

Max nTPM: 100.1
Min nTPM: 93.6

Sample A		94.7
Sample B		93.6
Sample C		100.1
Sample D		97.3

PONS - Rat RNA-seq

Max subtype nTPMⁱ Max subtype nTPM is the value for the subtype with the largest average nTPM across the subtype samples.	80.2

Rat sample

Sample description

nTPM

Pons
nTPM: 80.2

Samples: 4

Max nTPM: 83.5
Min nTPM: 75.3

Sample A		79.0
Sample A		75.3
Sample A		83.5
Sample A		83.0