Single cell type - CASP8AP2 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	CASP8AP2
Gene descriptionⁱ Full gene name according to HGNC.	Caspase 8 associated protein 2
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location.	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	3

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity.	Non-specific - Mixed function (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected.	Cell type enhanced (Microglia, Podocytes, Distal convoluted tubule cells, Loop of henle epithelial cells, Oligodendrocytes, Proximal tubule cells, Choroid plexus epithelial cells, Renal collecting duct intercalated cells, Renal connecting tubule cells, Bergmann glia, Brain excitatory neurons, Brain inhibitory neurons, Renal collecting duct principal cells, Oligodendrocyte progenitor cells, Papillary tip epithelial cells, Astrocytes, Other brain neurons, Ependymal cells)
Tau specificity scoreⁱ Tau specificity score is a numerical indicator of the specificity of the gene expression across cells or tissues. The value ranges from 0 and 1, where 0 indicates identical expression across all cells/tissue types, while 1 indicates expression in a single cell/tissue type.	0.68
Brain cluster specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell cluster types based on snRNA-seq data from brain samples. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. "cell type" refer to one of the 34 cluster names used to summarize the 2.5 million cells included in the brain cell representation.	Low cell type specificity
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected.	Low immune cell specificity
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); neXtProt protein existence (neXtProt evidence);and a Human Protein Atlas antibody- or RNA based score (HPA evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable.	Evidence at transcript level

ASSAYSⁱ

Single cell type

Cell type enrichment

Single nuclei - Brain

Immune cells

Expression clustering

SINGLE CELL TYPESⁱ A summary of normalized single cell RNA (nCPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. * Single nuclei Read more
Single cell type groups RNA single cell type group specificity: Cell type enhanced (Glial cells, Renal nephron cells, Ciliated cells, Ductal cells, Neuronal cells)

TISSUE CELL TYPEⁱ The tissue cell type section provides a cell type specificity prediction for all protein coding genes. More information on the data available can be found here and a description of the analysis here.
	Core cell typesⁱ The core cell type table displays data for a panel of eight cell types that are found in many tissues. Colored dots indicate that the selected gene has core cell type specificity in the indicated tissue. Grey dots indicate that cell type is present within that tissue, but the selected gene is not predicted to be enriched there. Category Alphabetical Enrichment Tissue specific cell typesⁱ This plot shows the enrichment score of the selected gene in all non-core cell types found within the profiled tissues, allowing comparisons between different cell types within different tissues. An enlarged symbol indicates classification of the gene as cell type enriched in the corresponding cell type.

SINGLE NUCLEI BRAINⁱ

Single nuclei cluster types

RNA brain cluster specificity: Low cell type specificity

IMMUNE CELL TYPE EXPRESSION (RNA)ⁱ Blood cell type expression overview shows RNA-seq data from three different sources: Internally generated Human Protein Atlas HPA data, as well as data generated by Monaco et al and Schmiedel et al). Color-coding is based on blood cell type lineages, including B-cells, T-cells, NK-cells, monocytes, granulocytes and dendritic cells as well as total PBMC. To access sample data, click on tissue name or bar.
HPA datasetⁱ The resulting transcript expression values calculated as nTPM, resulting from the internal normalization pipeline for 18 immune cell types and total peripheral blood mononuclear cells (PBMC). Lineage Expression Alphabetical Immune cell type specificity: Low immune cell specificity
Monaco datasetⁱ The transcript expression values (nTPM) resulting from the internal normalization pipeline are visualized for 29 blood cell types and total peripheral blood mononuclear cells (PBMC) from Monaco et al. Lineage Expression Alphabetical
Schmiedel datasetⁱ Transcript Per Million (TPM) values are visualized for 15 blood cell types from the Schmiedel et al. dataset. Lineage Expression Alphabetical

EXPRESSION CLUSTERING & CORRELATIONⁱ

SINGLE CELL TYPE

CASP8AP2 is part of cluster 102 Non-specific - Mixed function with confidenceⁱ 1
284 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data.	Clusterⁱ ID of the expression cluster of the neighboring gene.
INO80D	INO80 complex subunit D	0.8601	102
NUP160	Nucleoporin 160	0.8457	102
LSM14A	LSM14A mRNA processing body assembly factor	0.8262	102
WDFY1	WD repeat and FYVE domain containing 1	0.7933	102
PARN	Poly(A)-specific ribonuclease	0.7930	102
PEX26	Peroxisomal biogenesis factor 26	0.7925	102
RBSN	Rabenosyn, RAB effector	0.7921	102
RRN3	RRN3 homolog, RNA polymerase I transcription factor	0.7831	102
PHRF1	PHD and ring finger domains 1	0.7651	102
FNBP4	Formin binding protein 4	0.7643	102
MARF1	Meiosis regulator and mRNA stability factor 1	0.7623	102
KANSL1	KAT8 regulatory NSL complex subunit 1	0.7582	102
KAT6B	Lysine acetyltransferase 6B	0.7574	102
MAML1	Mastermind like transcriptional coactivator 1	0.7573	102
HERC2	HECT and RLD domain containing E3 ubiquitin protein ligase 2	0.7514	102

IMMUNE CELLS

CASP8AP2 is part of cluster 41 Basophils - Unknown function with confidenceⁱ 0.95
293 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data.	Clusterⁱ ID of the expression cluster of the neighboring gene.
ZNF358	Zinc finger protein 358	1.0000	41
AKR1C3	Aldo-keto reductase family 1 member C3	1.0000	41
BRD7	Bromodomain containing 7	1.0000	41
CD244	CD244 molecule	0.9762	41
FAM149B1	Family with sequence similarity 149 member B1	0.9762	41
AHI1	Abelson helper integration site 1	0.9762	41
AP4B1	Adaptor related protein complex 4 subunit beta 1	0.9762	41
ZMYM5	Zinc finger MYM-type containing 5	0.9762	41
PRKAB2	Protein kinase AMP-activated non-catalytic subunit beta 2	0.9762	41
GALNT15	Polypeptide N-acetylgalactosaminyltransferase 15	0.9762	41
RSBN1	Round spermatid basic protein 1	0.9762	41
DLST	Dihydrolipoamide S-succinyltransferase	0.9762	41
TRIM37	Tripartite motif containing 37	0.9762	41
ATP8B4	ATPase phospholipid transporting 8B4 (putative)	0.9762	41
BEX4	Brain expressed X-linked 4	0.9762	41