Single cell type - PYURF - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	PYURF
Gene descriptionⁱ Full gene name according to HGNC.	PIGY upstream open reading frame
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Disease related genes Essential proteins Metabolic proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location.	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	1

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity.	Renal epithelial cells - Ion transport & homeostasis (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected.	Group enriched (Papillary tip epithelial cells, Podocytes, Renal collecting duct principal cells, Loop of henle epithelial cells, Renal collecting duct intercalated cells, Distal convoluted tubule cells, Renal connecting tubule cells, Proximal tubule cells)
Tau specificity scoreⁱ Tau specificity score is a numerical indicator of the specificity of the gene expression across cells or tissues. The value ranges from 0 and 1, where 0 indicates identical expression across all cells/tissue types, while 1 indicates expression in a single cell/tissue type.	0.95
Brain cluster specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell cluster types based on snRNA-seq data from brain samples. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. "cell type" refer to one of the 34 cluster names used to summarize the 2.5 million cells included in the brain cell representation.	Not available
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected.	Low immune cell specificity
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); neXtProt protein existence (neXtProt evidence);and a Human Protein Atlas antibody- or RNA based score (HPA evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable.	Evidence at protein level

ASSAYSⁱ

Single cell type

Immune cells

Expression clustering

SINGLE CELL TYPESⁱ A summary of normalized single cell RNA (nCPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. * Single nuclei Read more
Single cell type groups RNA single cell type group specificity: Group enriched (Renal nephron cells, Ductal cells)

IMMUNE CELL TYPE EXPRESSION (RNA)ⁱ Blood cell type expression overview shows RNA-seq data from three different sources: Internally generated Human Protein Atlas HPA data, as well as data generated by Monaco et al and Schmiedel et al). Color-coding is based on blood cell type lineages, including B-cells, T-cells, NK-cells, monocytes, granulocytes and dendritic cells as well as total PBMC. To access sample data, click on tissue name or bar.
HPA datasetⁱ The resulting transcript expression values calculated as nTPM, resulting from the internal normalization pipeline for 18 immune cell types and total peripheral blood mononuclear cells (PBMC). Lineage Expression Alphabetical Immune cell type specificity: Low immune cell specificity
Monaco datasetⁱ The transcript expression values (nTPM) resulting from the internal normalization pipeline are visualized for 29 blood cell types and total peripheral blood mononuclear cells (PBMC) from Monaco et al. Lineage Expression Alphabetical
Schmiedel datasetⁱ Transcript Per Million (TPM) values are visualized for 15 blood cell types from the Schmiedel et al. dataset. Lineage Expression Alphabetical

EXPRESSION CLUSTERING & CORRELATIONⁱ

SINGLE CELL TYPE

PYURF is part of cluster 64 Renal epithelial cells - Ion transport & homeostasis with confidenceⁱ 1
105 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data.	Clusterⁱ ID of the expression cluster of the neighboring gene.
FAM50B	Family with sequence similarity 50 member B	0.8799	64
AQP2	Aquaporin 2	0.8082	64
RAB13	RAB13, member RAS oncogene family	0.8058	64
FXYD4	FXYD domain containing ion transport regulator 4	0.7940	64
ENSG00000285085	Novel protein	0.7877	64
UTP14C	UTP14C small subunit processome component	0.7874	105
WNT9B	Wnt family member 9B	0.7820	64
RNF5	Ring finger protein 5	0.7795	64
AGRN	Agrin	0.7703	64
TMEM238L	Transmembrane protein 238 like	0.7656	64
CCDC71L	Coiled-coil domain containing 71 like	0.7625	105
HS3ST6	Heparan sulfate-glucosamine 3-sulfotransferase 6	0.7507	64
ZNF90	Zinc finger protein 90	0.7402	64
IFNE	Interferon epsilon	0.7399	64
SLC14A2	Solute carrier family 14 member 2	0.7381	64

IMMUNE CELLS

PYURF is part of cluster 9 T-regs - Cell cycle regulation with confidenceⁱ 0.89
166 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data.	Clusterⁱ ID of the expression cluster of the neighboring gene.
PIGY	Phosphatidylinositol glycan anchor biosynthesis class Y	1.0000	9
HARS1	Histidyl-tRNA synthetase 1	1.0000	9
TRAF1	TNF receptor associated factor 1	0.9762	9
ADH5	Alcohol dehydrogenase 5 (class III), chi polypeptide	0.9762	5
GIPC1	GIPC PDZ domain containing family member 1	0.9762	9
REXO2	RNA exonuclease 2	0.9524	9
PBX4	PBX homeobox 4	0.9524	9
NPDC1	Neural proliferation, differentiation and control 1	0.9524	9
PDCD1	Programmed cell death 1	0.9524	9
CHN1	Chimerin 1	0.9524	9
ACVR1	Activin A receptor type 1	0.9286	9
TMEM35B	Transmembrane protein 35B	0.9286	5
TMEM116	Transmembrane protein 116	0.9286	5
CDR2	Cerebellar degeneration related protein 2	0.9286	6
ZNF330	Zinc finger protein 330	0.9286	6