Single cell type - HTR7 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	HTR7
Gene descriptionⁱ Full gene name according to HGNC.	5-hydroxytryptamine receptor 7
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	FDA approved drug targets G-protein coupled receptors RAS pathway related proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location.	Membrane
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	3

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity.	Neurons - Neuronal signaling (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected.	Cell type enhanced (Hepatic stellate cells, CDC, Macrophages, Undifferentiated spermatogonia, Other brain neurons, Brain excitatory neurons, Late primary spermatocytes, Brain inhibitory neurons, Monocytes, Hofbauer cells)
Tau specificity scoreⁱ Tau specificity score is a numerical indicator of the specificity of the gene expression across cells or tissues. The value ranges from 0 and 1, where 0 indicates identical expression across all cells/tissue types, while 1 indicates expression in a single cell/tissue type.	0.85
Brain cluster specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell cluster types based on snRNA-seq data from brain samples. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. "cell type" refer to one of the 34 cluster names used to summarize the 2.5 million cells included in the brain cell representation.	Cell type enhanced (Mammillary body, LAMP5-LHX6 and Chandelier)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected.	Immune cell enriched (myeloid DC)
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); neXtProt protein existence (neXtProt evidence);and a Human Protein Atlas antibody- or RNA based score (HPA evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable.	Evidence at protein level

ASSAYSⁱ

Single cell type

Cell type enrichment

Single nuclei - Brain

Immune cells

Expression clustering

SINGLE CELL TYPESⁱ A summary of normalized single cell RNA (nCPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. * Single nuclei Read more
Single cell type groups RNA single cell type group specificity: Group enriched (Stellate cells, Dendritic cells, Mononuclear phagocytes, Spermatogenic cell types, Neuronal cells)

TISSUE CELL TYPEⁱ The tissue cell type section provides a cell type specificity prediction for all protein coding genes. More information on the data available can be found here and a description of the analysis here.
	Core cell typesⁱ The core cell type table displays data for a panel of eight cell types that are found in many tissues. Colored dots indicate that the selected gene has core cell type specificity in the indicated tissue. Grey dots indicate that cell type is present within that tissue, but the selected gene is not predicted to be enriched there. Category Alphabetical Enrichment Tissue specific cell typesⁱ This plot shows the enrichment score of the selected gene in all non-core cell types found within the profiled tissues, allowing comparisons between different cell types within different tissues. An enlarged symbol indicates classification of the gene as cell type enriched in the corresponding cell type.

SINGLE NUCLEI BRAINⁱ

Single nuclei cluster types

RNA brain cluster specificity: Cell type enhanced (Mammillary body, LAMP5-LHX6 and Chandelier)

IMMUNE CELL TYPE EXPRESSION (RNA)ⁱ Blood cell type expression overview shows RNA-seq data from three different sources: Internally generated Human Protein Atlas HPA data, as well as data generated by Monaco et al and Schmiedel et al). Color-coding is based on blood cell type lineages, including B-cells, T-cells, NK-cells, monocytes, granulocytes and dendritic cells as well as total PBMC. To access sample data, click on tissue name or bar.
HPA datasetⁱ The resulting transcript expression values calculated as nTPM, resulting from the internal normalization pipeline for 18 immune cell types and total peripheral blood mononuclear cells (PBMC). Lineage Expression Alphabetical Immune cell type specificity: Immune cell enriched (myeloid DC)
Monaco datasetⁱ The transcript expression values (nTPM) resulting from the internal normalization pipeline are visualized for 29 blood cell types and total peripheral blood mononuclear cells (PBMC) from Monaco et al. Lineage Expression Alphabetical
Schmiedel datasetⁱ Transcript Per Million (TPM) values are visualized for 15 blood cell types from the Schmiedel et al. dataset. Lineage Expression Alphabetical

EXPRESSION CLUSTERING & CORRELATIONⁱ

SINGLE CELL TYPE

HTR7 is part of cluster 109 Neurons - Neuronal signaling with confidenceⁱ 1
333 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data.	Clusterⁱ ID of the expression cluster of the neighboring gene.
TRPC5	Transient receptor potential cation channel subfamily C member 5	0.6023	109
HCRTR2	Hypocretin receptor 2	0.5593	109
OR2M3	Olfactory receptor family 2 subfamily M member 3	0.5530	109
ELAVL2	ELAV like RNA binding protein 2	0.5385	109
TAS2R9	Taste 2 receptor member 9	0.5289	109
OR2M2	Olfactory receptor family 2 subfamily M member 2	0.5253	109
TAS2R7	Taste 2 receptor member 7	0.5121	109
OR2M4	Olfactory receptor family 2 subfamily M member 4	0.5118	109
CLEC2L	C-type lectin domain family 2 member L	0.4931	109
HHLA1	HERV-H LTR-associating 1	0.4847	109
OR2T6	Olfactory receptor family 2 subfamily T member 6	0.4822	109
ASNS	Asparagine synthetase (glutamine-hydrolyzing)	0.4803	98
LGI2	Leucine rich repeat LGI family member 2	0.4792	109
OC90	Otoconin 90	0.4770	109
ST8SIA2	ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2	0.4731	109

IMMUNE CELLS

HTR7 is part of cluster 26 Monocytes - Plasma membrane proteins with confidenceⁱ 0.76
79 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data.	Clusterⁱ ID of the expression cluster of the neighboring gene.
VIM	Vimentin	1.0000	26
CRTAP	Cartilage associated protein	1.0000	26
MACROH2A2	MacroH2A.2 histone	1.0000	26
CD33	CD33 molecule	0.9762	26
ENSG00000257767	Novel protein	0.9762	26
TNFSF13	TNF superfamily member 13	0.9762	26
KCNK13	Potassium two pore domain channel subfamily K member 13	0.9762	26
C1orf43	Chromosome 1 open reading frame 43	0.9762	26
ADAM15	ADAM metallopeptidase domain 15	0.9762	26
NIBAN2	Niban apoptosis regulator 2	0.9762	26
GPD2	Glycerol-3-phosphate dehydrogenase 2	0.9762	26
ATP5F1B	ATP synthase F1 subunit beta	0.9762	26
OTUB2	OTU deubiquitinase, ubiquitin aldehyde binding 2	0.9762	26
PKM	Pyruvate kinase M1/2	0.9762	26
SLC22A16	Solute carrier family 22 member 16	0.9524	26