Single cell type - SESN3 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	SESN3
Gene descriptionⁱ Full gene name according to HGNC.	Sestrin 3
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location.	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	5

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity.	B-cells - Adaptive immunity: Humoral response (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected.	Cell type enhanced (Late spermatids, Early spermatids, Retinal pigment epithelial cells, Late primary spermatocytes, B-cells)
Tau specificity scoreⁱ Tau specificity score is a numerical indicator of the specificity of the gene expression across cells or tissues. The value ranges from 0 and 1, where 0 indicates identical expression across all cells/tissue types, while 1 indicates expression in a single cell/tissue type.	0.43
Brain cluster specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell cluster types based on snRNA-seq data from brain samples. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. "cell type" refer to one of the 34 cluster names used to summarize the 2.5 million cells included in the brain cell representation.	Cell type enhanced (Pericyte)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected.	Low immune cell specificity
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); neXtProt protein existence (neXtProt evidence);and a Human Protein Atlas antibody- or RNA based score (HPA evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable.	Evidence at protein level

ASSAYSⁱ

Single cell type

Cell type enrichment

Single nuclei - Brain

Immune cells

Expression clustering

SINGLE CELL TYPESⁱ A summary of normalized single cell RNA (nCPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. * Single nuclei Read more
Single cell type groups RNA single cell type group specificity: Cell type enhanced (Spermatogenic cell types, Pigment cells)

TISSUE CELL TYPEⁱ The tissue cell type section provides a cell type specificity prediction for all protein coding genes. More information on the data available can be found here and a description of the analysis here.
	Core cell typesⁱ The core cell type table displays data for a panel of eight cell types that are found in many tissues. Colored dots indicate that the selected gene has core cell type specificity in the indicated tissue. Grey dots indicate that cell type is present within that tissue, but the selected gene is not predicted to be enriched there. Category Alphabetical Enrichment Tissue specific cell typesⁱ This plot shows the enrichment score of the selected gene in all non-core cell types found within the profiled tissues, allowing comparisons between different cell types within different tissues. An enlarged symbol indicates classification of the gene as cell type enriched in the corresponding cell type.

SINGLE NUCLEI BRAINⁱ

Single nuclei cluster types

RNA brain cluster specificity: Cell type enhanced (Pericyte)

IMMUNE CELL TYPE EXPRESSION (RNA)ⁱ Blood cell type expression overview shows RNA-seq data from three different sources: Internally generated Human Protein Atlas HPA data, as well as data generated by Monaco et al and Schmiedel et al). Color-coding is based on blood cell type lineages, including B-cells, T-cells, NK-cells, monocytes, granulocytes and dendritic cells as well as total PBMC. To access sample data, click on tissue name or bar.
HPA datasetⁱ The resulting transcript expression values calculated as nTPM, resulting from the internal normalization pipeline for 18 immune cell types and total peripheral blood mononuclear cells (PBMC). Lineage Expression Alphabetical Immune cell type specificity: Low immune cell specificity
Monaco datasetⁱ The transcript expression values (nTPM) resulting from the internal normalization pipeline are visualized for 29 blood cell types and total peripheral blood mononuclear cells (PBMC) from Monaco et al. Lineage Expression Alphabetical
Schmiedel datasetⁱ Transcript Per Million (TPM) values are visualized for 15 blood cell types from the Schmiedel et al. dataset. Lineage Expression Alphabetical

EXPRESSION CLUSTERING & CORRELATIONⁱ

SINGLE CELL TYPE

SESN3 is part of cluster 87 B-cells - Adaptive immunity: Humoral response with confidenceⁱ 1
200 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data.	Clusterⁱ ID of the expression cluster of the neighboring gene.
CHMP7	Charged multivesicular body protein 7	0.5244	87
ATF7IP	Activating transcription factor 7 interacting protein	0.5191	87
NPAT	Nuclear protein, coactivator of histone transcription	0.4894	9
FCMR	Fc mu receptor	0.4811	87
C12orf42	Chromosome 12 open reading frame 42	0.4794	87
METTL4	Methyltransferase 4, N6-adenosine	0.4551	7
ZNF891	Zinc finger protein 891	0.4365	7
OR52N4	Olfactory receptor family 52 subfamily N member 4	0.4326	87
PIKFYVE	Phosphoinositide kinase, FYVE-type zinc finger containing	0.4304	87
PARP15	Poly(ADP-ribose) polymerase family member 15	0.4261	87
ZNF107	Zinc finger protein 107	0.4205	87
CEP120	Centrosomal protein 120	0.4203	9
OR56B2P	Olfactory receptor family 56 subfamily B member 2 pseudogene	0.4175	87
MYCBP2	MYC binding protein 2	0.4167	7
ARHGEF18	Rho/Rac guanine nucleotide exchange factor 18	0.4097	87

IMMUNE CELLS

SESN3 is part of cluster 16 Non-specific - Cell proliferation with confidenceⁱ 0.95
161 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data.	Clusterⁱ ID of the expression cluster of the neighboring gene.
FAN1	FANCD2 and FANCI associated nuclease 1	1.0000	16
POLR3E	RNA polymerase III subunit E	0.9762	16
ZSCAN22	Zinc finger and SCAN domain containing 22	0.9762	16
BBS1	Bardet-Biedl syndrome 1	0.9762	16
TUBB2A	Tubulin beta 2A class IIa	0.9762	16
EXOC6B	Exocyst complex component 6B	0.9762	16
NRIP2	Nuclear receptor interacting protein 2	0.9524	16
ANKS3	Ankyrin repeat and sterile alpha motif domain containing 3	0.9524	16
CDKAL1	CDK5 regulatory subunit associated protein 1 like 1	0.9524	16
TIFA	TRAF interacting protein with forkhead associated domain	0.9286	43
GEMIN6	Gem nuclear organelle associated protein 6	0.9286	33
TNFRSF14	TNF receptor superfamily member 14	0.9286	33
GNE	Glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase	0.9286	33
MECR	Mitochondrial trans-2-enoyl-CoA reductase	0.9286	16
URGCP	Upregulator of cell proliferation	0.9286	33