Single cell type - GFRA2 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	GFRA2
Gene descriptionⁱ Full gene name according to HGNC.	GDNF family receptor alpha 2
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location.	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	6

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity.	Macrophages - Phagocytosis & lysosomal degradation (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected.	Cell type enhanced (Retinal horizontal cells, Late spermatids, Early spermatids, Kupffer cells, Adrenal medulla cells, Epididymal efferent duct absorptive cells, CDC, Macrophages)
Tau specificity scoreⁱ Tau specificity score is a numerical indicator of the specificity of the gene expression across cells or tissues. The value ranges from 0 and 1, where 0 indicates identical expression across all cells/tissue types, while 1 indicates expression in a single cell/tissue type.	0.84
Brain cluster specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell cluster types based on snRNA-seq data from brain samples. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. "cell type" refer to one of the 34 cluster names used to summarize the 2.5 million cells included in the brain cell representation.	Low cell type specificity
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected.	Immune cell enhanced (intermediate monocyte)
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); neXtProt protein existence (neXtProt evidence);and a Human Protein Atlas antibody- or RNA based score (HPA evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable.	Evidence at protein level

ASSAYSⁱ

Single cell type

Cell type enrichment

Single nuclei - Brain

Immune cells

Expression clustering

SINGLE CELL TYPESⁱ A summary of normalized single cell RNA (nCPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. * Single nuclei Read more
Single cell type groups RNA single cell type group specificity: Group enriched (Neuronal cells, Spermatogenic cell types)

TISSUE CELL TYPEⁱ The tissue cell type section provides a cell type specificity prediction for all protein coding genes. More information on the data available can be found here and a description of the analysis here.
	Core cell typesⁱ The core cell type table displays data for a panel of eight cell types that are found in many tissues. Colored dots indicate that the selected gene has core cell type specificity in the indicated tissue. Grey dots indicate that cell type is present within that tissue, but the selected gene is not predicted to be enriched there. Category Alphabetical Enrichment Tissue specific cell typesⁱ This plot shows the enrichment score of the selected gene in all non-core cell types found within the profiled tissues, allowing comparisons between different cell types within different tissues. An enlarged symbol indicates classification of the gene as cell type enriched in the corresponding cell type.

SINGLE NUCLEI BRAINⁱ

Single nuclei cluster types

RNA brain cluster specificity: Low cell type specificity

IMMUNE CELL TYPE EXPRESSION (RNA)ⁱ Blood cell type expression overview shows RNA-seq data from three different sources: Internally generated Human Protein Atlas HPA data, as well as data generated by Monaco et al and Schmiedel et al). Color-coding is based on blood cell type lineages, including B-cells, T-cells, NK-cells, monocytes, granulocytes and dendritic cells as well as total PBMC. To access sample data, click on tissue name or bar.
HPA datasetⁱ The resulting transcript expression values calculated as nTPM, resulting from the internal normalization pipeline for 18 immune cell types and total peripheral blood mononuclear cells (PBMC). Lineage Expression Alphabetical Immune cell type specificity: Immune cell enhanced (intermediate monocyte)
Monaco datasetⁱ The transcript expression values (nTPM) resulting from the internal normalization pipeline are visualized for 29 blood cell types and total peripheral blood mononuclear cells (PBMC) from Monaco et al. Lineage Expression Alphabetical
Schmiedel datasetⁱ Transcript Per Million (TPM) values are visualized for 15 blood cell types from the Schmiedel et al. dataset. Lineage Expression Alphabetical

EXPRESSION CLUSTERING & CORRELATIONⁱ

SINGLE CELL TYPE

GFRA2 is part of cluster 72 Macrophages - Phagocytosis & lysosomal degradation with confidenceⁱ 1
247 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data.	Clusterⁱ ID of the expression cluster of the neighboring gene.
PKD2L1	Polycystin 2 like 1, transient receptor potential cation channel	0.5862	72
CD163L1	CD163 molecule like 1	0.5626	72
RGL1	Ral guanine nucleotide dissociation stimulator like 1	0.5423	72
RBPJ	Recombination signal binding protein for immunoglobulin kappa J region	0.5247	72
SIGLEC1	Sialic acid binding Ig like lectin 1	0.5068	72
CMKLR1	Chemerin chemokine-like receptor 1	0.5025	72
ADAP2	ArfGAP with dual PH domains 2	0.4965	72
ENSG00000289692	Novel protein	0.4821	72
SDC3	Syndecan 3	0.4783	72
LGMN	Legumain	0.4777	72
CACNG7	Calcium voltage-gated channel auxiliary subunit gamma 7	0.4517	109
MERTK	MER proto-oncogene, tyrosine kinase	0.4498	72
MTSS1	MTSS I-BAR domain containing 1	0.4489	72
RNF135	Ring finger protein 135	0.4468	72
TCERG1L	Transcription elongation regulator 1 like	0.4452	109

IMMUNE CELLS

GFRA2 is part of cluster 25 Non-specific - Unknown function with confidenceⁱ 0.89
355 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data.	Clusterⁱ ID of the expression cluster of the neighboring gene.
LGALS9	Galectin 9	1.0000	25
UBAC1	UBA domain containing 1	1.0000	25
AUP1	AUP1 lipid droplet regulating VLDL assembly factor	0.9762	25
RPS6KB2	Ribosomal protein S6 kinase B2	0.9762	25
S100Z	S100 calcium binding protein Z	0.9762	25
NSMF	NMDA receptor synaptonuclear signaling and neuronal migration factor	0.9762	25
SLC66A3	Solute carrier family 66 member 3	0.9762	25
SIGLEC11	Sialic acid binding Ig like lectin 11	0.9762	25
PDK4	Pyruvate dehydrogenase kinase 4	0.9762	25
DHRS7B	Dehydrogenase/reductase 7B	0.9762	25
PPP2R1A	Protein phosphatase 2 scaffold subunit Aalpha	0.9762	25
UGGT2	UDP-glucose glycoprotein glucosyltransferase 2	0.9762	25
ULK2	Unc-51 like autophagy activating kinase 2	0.9762	25
TBXAS1	Thromboxane A synthase 1	0.9762	25
ITGA4	Integrin subunit alpha 4	0.9524	25