Single cell type - MRPL53 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	MRPL53
Gene descriptionⁱ Full gene name according to HGNC.	Mitochondrial ribosomal protein L53
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Essential proteins Ribosomal proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location.	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	2

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity.	Non-specific - Protein processing (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected.	Cell type enhanced (Parietal cells, Epididymal principal cells, Enterocytes, Neuroendocrine cells, Epididymal efferent duct absorptive cells, Epididymal efferent duct ciliated cells, Colonocytes, Megakaryocytes, Goblet cells, Gastric chief cells, Mucous neck cells, Enteric transient amplifying cells)
Tau specificity scoreⁱ Tau specificity score is a numerical indicator of the specificity of the gene expression across cells or tissues. The value ranges from 0 and 1, where 0 indicates identical expression across all cells/tissue types, while 1 indicates expression in a single cell/tissue type.	0.71
Brain cluster specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell cluster types based on snRNA-seq data from brain samples. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. "cell type" refer to one of the 34 cluster names used to summarize the 2.5 million cells included in the brain cell representation.	Low cell type specificity
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected.	Low immune cell specificity
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); neXtProt protein existence (neXtProt evidence);and a Human Protein Atlas antibody- or RNA based score (HPA evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable.	Evidence at protein level

ASSAYSⁱ

Single cell type

Single nuclei - Brain

Immune cells

Expression clustering

SINGLE CELL TYPESⁱ A summary of normalized single cell RNA (nCPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. * Single nuclei Read more
Single cell type groups RNA single cell type group specificity: Cell type enhanced (Gastric glandular cells, Reproductive supporting cells)

SINGLE NUCLEI BRAINⁱ

Single nuclei cluster types

RNA brain cluster specificity: Low cell type specificity

IMMUNE CELL TYPE EXPRESSION (RNA)ⁱ Blood cell type expression overview shows RNA-seq data from three different sources: Internally generated Human Protein Atlas HPA data, as well as data generated by Monaco et al and Schmiedel et al). Color-coding is based on blood cell type lineages, including B-cells, T-cells, NK-cells, monocytes, granulocytes and dendritic cells as well as total PBMC. To access sample data, click on tissue name or bar.
HPA datasetⁱ The resulting transcript expression values calculated as nTPM, resulting from the internal normalization pipeline for 18 immune cell types and total peripheral blood mononuclear cells (PBMC). Lineage Expression Alphabetical Immune cell type specificity: Low immune cell specificity
Monaco datasetⁱ The transcript expression values (nTPM) resulting from the internal normalization pipeline are visualized for 29 blood cell types and total peripheral blood mononuclear cells (PBMC) from Monaco et al. Lineage Expression Alphabetical
Schmiedel datasetⁱ Transcript Per Million (TPM) values are visualized for 15 blood cell types from the Schmiedel et al. dataset. Lineage Expression Alphabetical

EXPRESSION CLUSTERING & CORRELATIONⁱ

SINGLE CELL TYPE

MRPL53 is part of cluster 15 Non-specific - Protein processing with confidenceⁱ 1
232 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data.	Clusterⁱ ID of the expression cluster of the neighboring gene.
SELENOH	Selenoprotein H	0.8430	15
GABARAP	GABA type A receptor-associated protein	0.8358	15
ARL6IP4	ADP ribosylation factor like GTPase 6 interacting protein 4	0.7875	15
NDUFA13	NADH:ubiquinone oxidoreductase subunit A13	0.7719	15
RNASEK-C17orf49	RNASEK-C17orf49 readthrough	0.7292	15
RPL17	Ribosomal protein L17	0.6738	15
ARPC4-TTLL3	ARPC4-TTLL3 readthrough	0.6611	15
NDUFV2	NADH:ubiquinone oxidoreductase core subunit V2	0.6567	15
DYNLRB1	Dynein light chain roadblock-type 1	0.6466	15
DHPS	Deoxyhypusine synthase	0.6356	15
SNRPD3	Small nuclear ribonucleoprotein D3 polypeptide	0.6349	15
RPS17	Ribosomal protein S17	0.6296	15
ENSG00000290318	Novel protein	0.6271	15
MICOS10	Mitochondrial contact site and cristae organizing system subunit 10	0.6084	15
OOEP	Oocyte expressed protein	0.5975	15

IMMUNE CELLS

MRPL53 is part of cluster 5 Non-specific - Cell proliferation with confidenceⁱ 0.66
689 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data.	Clusterⁱ ID of the expression cluster of the neighboring gene.
IFI27L2	Interferon alpha inducible protein 27 like 2	0.9524	5
ZNF576	Zinc finger protein 576	0.9524	5
MRPS11	Mitochondrial ribosomal protein S11	0.9524	5
CENPW	Centromere protein W	0.9048	13
CHCHD1	Coiled-coil-helix-coiled-coil-helix domain containing 1	0.9048	5
WRAP53	WD repeat containing antisense to TP53	0.9048	5
LRRC41	Leucine rich repeat containing 41	0.9048	10
N4BP2L1	NEDD4 binding protein 2 like 1	0.9048	10
MILR1	Mast cell immunoglobulin like receptor 1	0.8810	10
SULF2	Sulfatase 2	0.8810	10
MORN2	MORN repeat containing 2	0.8810	10
SMU1	SMU1 DNA replication regulator and spliceosomal factor	0.8810	5
PHYH	Phytanoyl-CoA 2-hydroxylase	0.8810	10
TIMM44	Translocase of inner mitochondrial membrane 44	0.8810	5
HSD17B4	Hydroxysteroid 17-beta dehydrogenase 4	0.8571	10