Cell Culture Engineering XVI
May 6, 2018 - May 11, 2018
Cell Culture Engineering conferences are held bi-annually. On May 6th Prof. Mathias Uhlen will open the conference with a keynote lecture on "The Human Protein Atlas - Implications for Human Biology, Drug Development and Precision Medicine". Preliminary programme
April 11, 2018, 6:00pm CEST, 6:00pm CEST
WEBINAR | Addressing the reproducibility crisis: Validating antibodies for life science research
Speakers: Mathias Uhlen and Cecilia Williams
Antibodies are invaluable tools in the life sciences. There are several challenges that must be overcome to validate the specificity and reproducibility of antibody reagents, as emphasized by many publications, including a recent proposal from the International Working Group for Antibody Validation (IWGAV). There are many examples of antibodies with off-target binding leading to erroneous conclusions, some of which will be discussed during this webcast, demonstrating the importance of application-specific validation. Five alternative strategies for enhanced validation of antibodies will be described based on genetic validation, recombinant expression, independent antibodies, orthogonal validation, and capture mass spectrometry validation. Approaches based on these five strategies have been used for streamlined, enhanced validation of more than 10,000 antibodies from more than 30 providers, targeting over 7,000 human proteins as part of the Human Protein Atlas program. The results show a path forward for validating antibodies in an application-specific manner suitable for both providers and users.
During the webinar, viewers will learn about:
- The generation and validation of data used in the Human Protein Atlas
- Problems related to the cross-reactivity of antibodies and the challenges they create for the interpretation of results
- Enhanced validation of antibodies using five application-specific strategies.
Speakers will answer your questions during the live broadcast!
Course in antibody based technologies at Scilifelab April
April 10, 2018 - April 11, 2018
Where: Air and Fire, Scilifelab Solna
When: April 10-11, 2018
Credits: 1 hp
The course will focus on antibody based technologies and describe the possibilities of using these technologies within the facilities at SciLifeLab. Lectures will cover both theoretical and more practical aspects of the different technologies, including the following:
- Tissue fluorescence profiling
- Protein array technologies and suspension bead technologies
- Mass cytometry and flow cytometry
- Immuno capture/immuno precipitation in combination with mass spectrometry
- Proximity ligation assays
Apart from general aspects of the technologies, the course will include project presentations to demonstrate the benefits and possibilities of the technologies in different contexts. Last but not least validation strategies of antibodies for different applications will be covered, as this is of outmost importance for adequate output data.
Please read more and apply through the following link:
Antibody based technologies
Bioprocessing Summit Europe
March 21, 2018 - March 22, 2018
On Thursday March 22nd, Prof Sophia Hober will present "A Protein A-Derived Domain with Calcium-Dependent Affinity for Use in Antibody Purification" at the Cambridge Healthtech Institute's Inaugural "Advances in Recovery and Purification" Programme.
March 11, 2018 - March 14, 2018
The title of 2018 year's US HUPO conference is "Technology accelerating discovery". Dr. Emma Lundberg will hold the closing plenary session, titled "Spatiotemporal proteome organization of the human cell" on March 14. Programme
Proteomics in Cell Biology and Disease Mechanisms
February 28, 2018 - March 2, 2018
The Wellcome Genome Campus organizes the conference Proteomics in Cell Biology and Disease Mechanisms where Dr. Emma Lundberg will present "Dissecting the spatiotemporal subcellular distribution of the human proteome". Her talk is scheduled on March 2. Draft programme
The Human Protein Atlas - implications for human biology and precision medicine
January 25, 2018
Dr Cecilia Lindskog will present the Pathology Atlas, the association of all human genes with patient survival, and other recent milestones and high-profile findings in the Human Protein Atlas project at the 8th Symposium on Pharmaceutical Profiling in Drug Discovery and Development. Venue: The Humanities Theater, English Park Campus, Uppsala University.
Frontiers in Biology
January 24, 2018
Upcoming presentation by Emma Lundberg at Stanford on "Spatial proteomics and the single cell".
Protein A-Based purification of antibodies
January 8, 2018
Prof. Sophia Hober will present the latest advances regarding engineering of protein A, for Ca-mediated purification of antibodies. Her talk is scheduled on January 9, during one of the largest annual events focusing on protein science, the PepTalk conference. "Optimization of an IgG-Binding, Protein A-Based Purification Matrix".
Presentation at Biogenouest network meeting
December 7, 2017
Dr. Cecilia Lindskog Bergström, Director of the Tissue Atlas, will present "The Human Protein Atlas - implications for human biology and precision medicine", at the annual meeting of the Biogenouest network in Nantes, France, this year with the theme "Antibodies used in proteomics".
ASCB/EMBO meeting starts on the 2 of December
December 2, 2017 - December 6, 2017
First joint ASCB/EMBO meeting is organized in Philadelphia in December. Invited talks highlight the value of sharing scientific results through publicly available databases and international co-operations to increase our understanding of cell biology. The Cell Atlas team presents protein atlas, in booth #218.
The HPA at the Image-based Cell Atlas Meeting
October 6, 2017 - November 30, 2017
World-leading scientists summoned to discuss how to map the human cell and promote research on human health and diseases. The meeting emphasised the importance of image-based assays to accomplish such an endeavor.