MYLK

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA031677

 

Antibody CAB020789

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 SDIX
 

Product name

HPA031677 3218.00.02 

Host species

Rabbit Rabbit 

Clonality

pAb msAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity 

Released in version

8 5 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Genetic immunization 

Length (aa)

145  

Antigen sequence

KCVAKNDAGQAECSCQVTVDDAPASENTKAPEMKSRRPKSSLPPVLGTES
DATVKKKPAPKTPPKAAMPPQIIQFPEDQKVRAGESVELFGKVTGTQPIT
CTWMKFRKQIQESEHMKVENSENGSKLTILAARQEHCGCYTLLVE
 
 

Matching transcripts

MYLK-001 - ENSP00000354004 [100%]
MYLK-003 - ENSP00000320622 [100%]
MYLK-004 - ENSP00000418335 [100%]
MYLK-201 - ENSP00000346846 [100%]
MYLK-202 - ENSP00000352088 [100%]
MYLK-203 - ENSP00000353452 [100%]
  

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.
More information
 Immunohistochemical staining of human smooth muscle shows moderate cytoplasmic positivity in smooth muscle cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:1500 1:550 

Literature conformity

Consistent with extensive gene/protein characterization data Consistent with extensive gene/protein characterization data 

RNA consistency

Mainly consistent with RNA expression data Mainly consistent with RNA expression data 

         Immunofluorescence

Image

  

Description

Application not done for this antibody. Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm.
More information
 

Antibody dilution

 1:137 

Validation IF

 Supportive: The subcellular location is supported by experimental gene/protein characterization data, gene silencing, or an independent antibody. 

         Western Blot

Image

  

Description

  

Target mass (kDa)

210.7, 205.1, 203.1 210.7, 205.1, 203.1, 25.3, 17.0, 16.8 

Antibody dilution

1:250 1:500 

Validation WB

Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Application not done for this antibody. 

Antibody dilution

1:3000  

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen.  
 

ANTIGEN VIEW

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MYLK-001
 
MYLK-003
 
MYLK-004
 
MYLK-201
 
MYLK-202
 
MYLK-203