On the welcome page of the dictionary, four major sections are shown: Normal tissues, Cancer, Cell structure and Protein expression. Below the image of each section are links to introductory texts for i) normal tissue histology, ii) hallmarks of cancer and iii) cell cycle overview. Within each section there are direct links to histology descriptions of different tissue types and tumor forms as well as descriptions of cell structures. Furthest to the right there is a "Protein expression" section which includes a list of genes with links to examples of different protein expression patterns.
A common feature for all sections is that a general descriptive text about the tissue, tumor-type, cell structure or protein/antibody is provided when browsing a particular topic.
The aim of the dictionary is to facilitate the interpretation and use of the image-based data available in the Human Protein Atlas, but also to serve as a tool for training and understanding tissue histology, pathology and cell biology. The dictionary contains three main parts, normal tissue histology, pathology and cell structure. This dictionary covering normal tissues and pathology is based on full tissue sections stained with hematoxylin-eosin (HE) and representative high-resolution images are displayed at different magnifications. The cell atlas covers intra-cellular structures and is based on immunofluorescence and confocal microscopy images, using different color channels to highlight the organelle structure of a cell.
A fourth part of this dictionary, protein expression, shows examples of immunohistochemistry and demonstrates how well-known proteins are expressed in various cells of different tissues.
Complementary annotation dictionaries using antibodies found in the Human Protein Atlas are available as PDF-documents:
Normal and cancer tissues have been stained using immunohistochemistry to visualize various protein expression patterns. Antibodies have been selected to demonstrate the expression patterns of well-known proteins and to reflect antibodies used in clinical diagnostics to determine the nature of a given cancer. For certain antibodies the corresponding protein expression pattern is shown in both normal and cancer tissues. For each exemplified protein there is a short descriptive text including the clinical usefulness of the corresponding antibody.
Smooth muscle marker
Marker for prostate cancer
Amylase - Marker for salivary gland and pancreas
Marker for antigen presenting cells
CD3 - General T cell marker
T helper cell marker
Marker for the reticular network in lymphatic tissues
General monocyte/histiocyte/macrophage marker
General B cell marker
CD8 - T cytotoxic cell marker
E-cadherin - Marker for non-neural epithelial cells
Marker for cells in the gastrointestinal tract
Chromogranin A - Marker for neuroendocrine cells
Desmin - Marker for muscle cells
HER2 - Treatment predictive marker for cancer
Estrogen receptor alpha - Marker for female tissues and cancers
Filaggrin - Differentiation marker for squamous epithelia
Gastrin - Marker for G-cells of the stomach
Glucagon - Marker for pancreatic alpha-cells
Glial fibrillary acidic protein - Marker for astrocytes
Insulin - Marker for pancreatic beta-cells
PSA - Marker for normal prostate and prostate cancer
Keratin 18 - Epithelial cell marker used for differential diagnostics of cancer
Keratin 19 - Epithelial cell marker used for differential diagnostics of cancer
Keratin 20 - Epithelial cell marker used for differential diagnostics of cancer
Keratin 6 (KRT6) - Epithelial cell marker used for differential diagnostics of cancer
Ki-67 - General marker for proliferation
Melan-A - Marker for normal melanocytes and melanoma
CD10 - Marker for acute lymphoblastic leukemia
NK2 homeobox 1 (TTF1) - Marker for lungcancer
CD31 - Marker for blood vessels
Progesterone receptor - Marker for female tissues and cancers
Marker for podocytes in glomeruli
Parathyroid hormone - Marker for the parathyroid gland
CD45 - General marker for hematopoietic and immune cells.
S100 - Marker for neurogenic and melanocytic cells
Marker for colorectal cancer
CD138 - General plasma cell marker
Somatostatin - Marker for pancreatic delta-cells
Thyroglobulin - Marker for normal thyroid and thyroid cancer
Troponin T type 3 - Marker for fast skeletal muscles
Tumor protein p53 - The anti-cancer watchdog
P63 - Marker for basal epithelial cells
Vimentin - Marker for mesenchymal cells
Immunohistochemistry annotation dictionary: screen usage (15MB), printing (95MB).