GRIPAP1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA000282

 

Antibody HPA000615

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA000282 HPA000615 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

1 1 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

115 115 

Antigen sequence

LKQCREQHAAELKGKEEELQDVRDQLEQAQEERDCHLKTISSLKQEVKDT
VDGQRILEKKGSAALKDLKRQLHLERKRADKLQERLQDILTNSKSRSGLE
ELVLSEMNSPSRTQT
 
KESSAVPARSLSSSPQAQPPRPAELSDEEVAELFQRLAETQQEKWMLEEK
VKHLEVSSASMAEDLCRKSAIIETYVMDSRIDVSVAAGHTDRSGLGSVLR
DLVKPGDENLREMNK
 

Matching transcripts

GRIPAP1-001 - ENSP00000365624 [100%]
GRIPAP1-005 - ENSP00000365608 [100%]
GRIPAP1-006 - ENSP00000365627 [100%]
GRIPAP1-004 - ENSP00000365606 [90%]
 GRIPAP1-001 - ENSP00000365624 [100%]
GRIPAP1-005 - ENSP00000365608 [100%]
GRIPAP1-006 - ENSP00000365627 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human gall bladder shows cytoplasmic positivity in glandular cells.
More information
 Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in islet cells.
More information
 

Retrieval method

HIER pH9 HIER pH6 

Antibody dilution

1:1500 1:100 

Literature conformity

Partly consistent with extensive gene/protein characterization data Partly consistent with extensive gene/protein characterization data 

RNA consistency

Not done Not done 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402754)
More information
 

Target mass (kDa)

96.0, 92.7, 90.8, 71.9 96.0, 92.7, 90.8 

Antibody dilution

1:500 1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:2000 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). Supportive: Pass with single peak corresponding to interaction only with its own antigen. 

         Immunofluorescence

Image

  

Description

Application not done for this antibody. Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm & vesicles.
More information
 

Antibody dilution

 1:40 

Validation IF

 Uncertain: The subcellular location is partly supported by literature or no literature is available. 
 

ANTIGEN VIEW

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GRIPAP1-001
 
GRIPAP1-004
 
GRIPAP1-005
 
GRIPAP1-006